A particulate platform for bioluminescent immunosensing

• The present study examines pyruvate kinase-conjugated antibodies for potential use in EUSA applications. The conjugates had an acceptable stability, and the coupling inflicted only minor impairment on the kinase activity. To mimic the setup of an immunoassay under development, a test antigen (BSA) was attached to polystyrene nanoparticles. This arrangement was found to be suitable as solid support for presentation of antigens in sensitive bioluminescence assays. The nanoparticles were well characterized in terms of protein surface load and were used to establish the number of conjugate complexes needed to generate a detectable signal. Under the biochemical conditions employed here, the detection limit of the pyruvate kinase conjugate lies in the femtomole range.

Ämnesord

NATURVETENSKAP  -- Kemi (hsv//swe)

NATURAL SCIENCES  -- Chemical Sciences (hsv//eng)

Nyckelord

Enzyme

Transferases

Biosensor

Chemical sensor

Pyruvate kinase

Detection limit

Protein

Chemical analysis

Bioluminescence

Nanoparticle

Immunological method

Stability

Antibody

Immunosensor

Chemistry

Kemi

Publikations- och innehållstyp

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