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  • Najjari, AfefTunis El Manar University (author)

Physiological and genomic insights into abiotic stress of halophilic archaeon Natrinema altunense 4.1R isolated from a saline ecosystem of Tunisian desert

  • Article/chapterEnglish2023

Publisher, publication year, extent ...

  • 2023-02-16
  • Springer Science and Business Media LLC,2023
  • 20 s.

Numbers

  • LIBRIS-ID:oai:lup.lub.lu.se:d951b6b1-d703-41a3-bb0a-6714d5669408
  • https://lup.lub.lu.se/record/d951b6b1-d703-41a3-bb0a-6714d5669408URI
  • https://doi.org/10.1007/s10709-023-00182-0DOI

Supplementary language notes

  • Language:English
  • Summary in:English

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  • Subject category:art swepub-publicationtype
  • Subject category:ref swepub-contenttype

Notes

  • Halophilic archaea are polyextremophiles with the ability to withstand fluctuations in salinity, high levels of ultraviolet radiation, and oxidative stress, allowing them to survive in a wide range of environments and making them an excellent model for astrobiological research. Natrinema altunense 4.1R is a halophilic archaeon isolated from the endorheic saline lake systems, Sebkhas, located in arid and semi-arid regions of Tunisia. It is an ecosystem characterized by periodic flooding from subsurface groundwater and fluctuating salinities. Here, we assess the physiological responses and genomic characterization of N. altunense 4.1R to UV-C radiation, as well as osmotic and oxidative stresses. Results showed that the 4.1R strain is able to survive up to 36% of salinity, up to 180 J/m2 to UV-C radiation, and at 50 mM of H2O2, a resistance profile similar to Halobacterium salinarum, a strain often used as UV-C resistant model. In order to understand the genetic determinants of N. altunense 4.1R survival strategy, we sequenced and analyzed its genome. Results showed multiple gene copies of osmotic stress, oxidative stress, and DNA repair response mechanisms supporting its survivability at extreme salinities and radiations. Indeed, the 3D molecular structures of seven proteins related to responses to UV-C radiation (excinucleases UvrA, UvrB, and UvrC, and photolyase), saline stress (trehalose-6-phosphate synthase OtsA and trehalose-phosphatase OtsB), and oxidative stress (superoxide dismutase SOD) were constructed by homology modeling. This study extends the abiotic stress range for the species N. altunense and adds to the repertoire of UV and oxidative stress resistance genes generally known from haloarchaeon.

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Added entries (persons, corporate bodies, meetings, titles ...)

  • Boussetta, AyoubTunis El Manar University (author)
  • Youssef, NohaOklahoma State University, Stillwater (author)
  • Linares-Pastén, Javier A.Lund University,Lunds universitet,Bioteknik,Centrum för tillämpade biovetenskaper,Kemiska institutionen,Institutioner vid LTH,Lunds Tekniska Högskola,Biotechnology,Center for Applied Life Sciences,Department of Chemistry,Departments at LTH,Faculty of Engineering, LTH(Swepub:lu)biot-jlp (author)
  • Mahjoubi, MounaUniversity of Manouba (author)
  • Belloum, RahmaTunis El Manar University (author)
  • Sghaier, HaithamNational Center for Nuclear Sciences and Technology, Tunisia (author)
  • Cherif, AmeurUniversity of Manouba (author)
  • Ouzari, Hadda ImeneTunis El Manar University (author)
  • Tunis El Manar UniversityOklahoma State University, Stillwater (creator_code:org_t)

Related titles

  • In:Genetica: Springer Science and Business Media LLC151:2, s. 133-1521573-68570016-6707

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