Astrocytic and neuronal oxidative metabolism are coupled to the rate of glutamate–glutamine cycle in the tree shrew visual cortex

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Sonnay, SarahSwiss Federal Institute of Technology (författare)

Astrocytic and neuronal oxidative metabolism are coupled to the rate of glutamate–glutamine cycle in the tree shrew visual cortex

Artikel/kapitelEngelska2018

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2017-11-09
Wiley,2018
15 s.

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LIBRIS-ID:oai:lup.lub.lu.se:daf012d7-1304-4519-8bb1-ac82a34973b3
https://lup.lub.lu.se/record/daf012d7-1304-4519-8bb1-ac82a34973b3URI
https://doi.org/10.1002/glia.23259DOI

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Språk:engelska
Sammanfattning på:engelska

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Ämneskategori:ref swepub-contenttype

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Astrocytes play an important role in glutamatergic neurotransmission, namely by clearing synaptic glutamate and converting it into glutamine that is transferred back to neurons. The rate of this glutamate–glutamine cycle (VNT) has been proposed to couple to that of glucose utilization and of neuronal tricarboxylic acid (TCA) cycle. In this study, we tested the hypothesis that glutamatergic neurotransmission is also coupled to the TCA cycle rate in astrocytes. For that we investigated energy metabolism by means of magnetic resonance spectroscopy (MRS) in the primary visual cortex of tree shrews (Tupaia belangeri) under light isoflurane anesthesia at rest and during continuous visual stimulation. After identifying the activated cortical volume by blood oxygenation level-dependent functional magnetic resonance imaging, 1H MRS was performed to measure stimulation-induced variations in metabolite concentrations. Relative to baseline, stimulation of cortical activity for 20 min caused a reduction of glucose concentration by −0.34 ± 0.09 µmol/g (p < 0.001), as well as a −9% ± 1% decrease of the ratio of phosphocreatine-to-creatine (p < 0.05). Then 13C MRS during [1,6-13C]glucose infusion was employed to measure fluxes of energy metabolism. Stimulation of glutamatergic activity, as indicated by a 20% increase of VNT, resulted in increased TCA cycle rates in neurons by 12% ((VTCA n, p < 0.001), p < 0.001) and in astrocytes by 24% ((VTCA g, p = 0.007). We further observed linear relationships between VNT and both VTCA n and VTCA g. Altogether, these results suggest that in the tree shrew primary visual cortex glutamatergic neurotransmission is linked to overall glucose oxidation and to mitochondrial metabolism in both neurons and astrocytes.

Ämnesord och genrebeteckningar

MEDICIN OCH HÄLSOVETENSKAP Medicinska och farmaceutiska grundvetenskaper Neurovetenskaper hsv//swe
MEDICAL AND HEALTH SCIENCES Basic Medicine Neurosciences hsv//eng
C
cortical energy metabolism
fMRI
MRS
stimulation

Biuppslag (personer, institutioner, konferenser, titlar ...)

Poirot, JordanUniversity of Fribourg (författare)
Just, NathalieUniversity Hospital Münster (författare)
Clerc, Anne CatherineSwiss Federal Institute of Technology (författare)
Gruetter, RolfUniversity of Lausanne,University of Geneva,Swiss Federal Institute of Technology (författare)
Rainer, GregorUniversity of Fribourg (författare)
Duarte, João M.N.Lund University,Lunds universitet,Diabetes och hjärnans funktion,Forskargrupper vid Lunds universitet,Diabetes and Brain Function,Lund University Research Groups,Swiss Federal Institute of Technology(Swepub:lu)jo2654du (författare)
Swiss Federal Institute of TechnologyUniversity of Fribourg (creator_code:org_t)

Sammanhörande titlar

Ingår i:GLIA: Wiley66:3, s. 477-4910894-1491

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Av författaren/redakt...: Sonnay, Sarah; Poirot, Jordan; Just, Nathalie; Clerc, Anne Cath ...; Gruetter, Rolf; Rainer, Gregor; visa fler...; Duarte, João M.N ...; visa färre...

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MEDICIN OCH HÄLSOVETENSKAP: MEDICIN OCH HÄLS ...; och Medicinska och f ...; och Neurovetenskaper

Artiklar i publikationen: GLIA

Av lärosätet: Lunds universitet

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