Association of erythroid transcription factors : complexes involving the LIM protein RBTN2 and the zinc-finger protein GATA1

• The RBTN2 LIM-domain protein, originally identified as an oncogenic protein in human T-cell leukemia, is essential for erythropoiesis. A possible role for RBTN2 in transcription during erythropoiesis has been investigated. Direct interaction of the RBTN2 protein was observed in vivo and in vitro with the GATA1 or -2 zinc-finger transcription factors, as well as with the basic helix-loop-helix protein TAL1. By using mammalian two-hybrid analysis, complexes involving RBTN2, TAL1, and GATA1, together with E47, the basic helix-loop-helix heterodimerization partner of TAL1, could be demonstrated. Thus, a molecular link exists between three proteins crucial for erythropoiesis, and the data suggest that variations in amounts of complexes involving RBTN2, TAL1, and GATA1 could be important for erythroid differentiation.

Subject headings

MEDICIN OCH HÄLSOVETENSKAP  -- Klinisk medicin -- Hematologi (hsv//swe)

MEDICAL AND HEALTH SCIENCES  -- Clinical Medicine -- Hematology (hsv//eng)

Keyword

Adaptor Proteins, Signal Transducing

Amino Acid Sequence

Animals

Basic Helix-Loop-Helix Transcription Factors

DNA-Binding Proteins

Erythroid-Specific DNA-Binding Factors

Erythropoiesis

GATA1 Transcription Factor

Helix-Loop-Helix Motifs

Homeodomain Proteins

LIM Domain Proteins

Leukemia, Erythroblastic, Acute

Metalloproteins

Mice

Molecular Sequence Data

Protein Binding

Proto-Oncogene Proteins

Recombinant Proteins

TCF Transcription Factors

Transcription Factor 7-Like 1 Protein

Transcription Factors

Tumor Cells, Cultured

Zinc Fingers

Publication and Content Type

art (subject category)

ref (subject category)