Summary report of the TD-3 workshop: characterization of 83 antibodies against prostate-specific antigen

Twelve research groups participated in the ISOBM TD-3 Workshop in which the reactivity and specificity of 83 antibodies against prostate-specific antigen (PSA) were investigated. Using a variety of techniques including cross-inhibition assays, Western blotting, BIAcore, immunoradiometric assays and immunohistochemistry, the antibodies were categorized into six major groups which formed the basis for mapping onto two- and three-dimensional (2-D and 3-D) models of PSA. The overall findings of the TD-3 Workshop are summarized in this report. In agreement with all participating groups, three main antigenic domains were identified: free PSA-specific epitopes located in or close to amino acids 86-91; discontinuous epitopes specific for PSA without human kallikrein (hK2) cross-reactivity located at or close to amino acids 158-163; and continuous or linear epitopes shared between PSA and hK2 located close to amino acids 3-11. In addition, several minor and partly overlapping domains were also identified. Clearly, the characterization of antibodies from this workshop and the location of their epitopes on the 3-D model of PSA illustrate the importance of selecting appropriate antibody pairs for use in immunoassays. It is hoped that these findings and the epitope nomenclature described in this TD-3 Workshop are used as a standard for future evaluation of anti-PSA antibodies.

Paus, E (author)
Allard, W J (author)
Andersson, I (author)
Andres, C (author)
Barnett, T R (author)
Becker, CharlotteLund University,Lunds universitet,Klinisk kemi, Malmö,Forskargrupper vid Lunds universitet,Clinical Chemistry, Malmö,Lund University Research Groups(Swepub:lu)klke-cbe (author)
Belenky, A (author)
Bellanger, L (author)
Pellegrino, C M (author)
Bormer, O P (author)
Davis, G (author)
Dowell, B (author)
Grauer, L S (author)
Jette, D C (author)
Karlsson, B (author)
Kreutz, F T (author)
van der Kwast, T M (author)
Lauren, L (author)
Leinimaa, M (author)
Leinonen, J (author)
Lilja, HansLund University,Lunds universitet,Klinisk kemi, Malmö,Forskargrupper vid Lunds universitet,Clinical Chemistry, Malmö,Lund University Research Groups(Swepub:lu)klke-hli (author)
Linton, H J (author)
Nap, M (author)
Nilsson, O (author)
Ng, P C (author)
Nustad, K (author)
Peter, A (author)
Pettersson, K (author)
Piironen, T (author)
Rapp, J (author)
Rittenhouse, H G (author)
Rye, P D (author)
Seguin, P (author)
Slota, J (author)
Sokoloff, R L (author)
Suresh, M R (author)
Very, D L (author)
Wang, T J (author)
Wigheden, IngridLund University,Lunds universitet,Celiaki och diabetes,Forskargrupper vid Lunds universitet,Celiac Disease and Diabetes Unit,Lund University Research Groups(Swepub:lu)klke-iwi (author)
Wolfert, R L (author)
Yeung, K K (author)
Zhang, W-M (author)
Zhou, Z (author)
Hilgers, J (author)
Klinisk kemi, MalmöForskargrupper vid Lunds universitet (creator_code:org_t)

In:Tumor Biology: Springer Science and Business Media LLC20:Suppl. 1, s. 1-121423-03801010-4283

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