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  • Hoogduijn, Martin JErasmus University Medical Center (författare)

Effects of freeze-thawing and intravenous infusion on mesenchymal stromal cell gene expression.

  • Artikel/kapitelEngelska2016

Förlag, utgivningsår, omfång ...

  • Mary Ann Liebert Inc,2016

Nummerbeteckningar

  • LIBRIS-ID:oai:lup.lub.lu.se:ddf799a0-0d4a-447c-bc69-6890d599f314
  • https://lup.lub.lu.se/record/8821686URI
  • https://doi.org/10.1089/scd.2015.0329DOI
  • http://kipublications.ki.se/Default.aspx?queryparsed=id:133361057URI

Kompletterande språkuppgifter

  • Språk:engelska
  • Sammanfattning på:engelska

Ingår i deldatabas

Klassifikation

  • Ämneskategori:art swepub-publicationtype
  • Ämneskategori:ref swepub-contenttype

Anmärkningar

  • Mesenchymal stromal cells (MSC) are increasingly used as an investigative therapeutic product for immune disorders and degenerative disease. Typically, MSC are isolated from human tissue, expanded in culture and cryopreserved until usage. The safety and efficacy of MSC therapy will depend on the phenotypical and functional characteristics of MSC. The freeze-thawing procedure may change these characteristics. Furthermore, the cells encounter a microenvironment after administration that may impact their properties. It has been demonstrated that the majority of MSC localize to the lungs after intravenous infusion, making this the site to study the effects of the in vivo milieu on administered MSC. In the present study we investigated the effect of freeze-thawing and the mouse lung microenvironment on human adipose tissue-derived MSC. There were effects of freeze-thawing on the whole genome expression profile of MSC, although the effects did not exceed inter-donor differences. There were no major changes in the expression of hemostatic regulators on transcriptional level, but significantly increased expression of procoagulant tissue factor on the surface of thawed adipose MSC, correlating with increased procoagulant activity of thawed cells. Exposure for 2h to the lung microenvironment had a major effect on MSC gene expression and affected several immunological pathways. This indicates that MSC undergo functional changes shortly after infusion and this may influence the efficacy of MSC to modulate inflammatory responses. The results of this study demonstrate that MSC rapidly alter in response to the local milieu and that disease specific conditions may shape MSC after administration.

Ämnesord och genrebeteckningar

Biuppslag (personer, institutioner, konferenser, titlar ...)

  • de Witte, SamanthaErasmus University Medical Center (författare)
  • Luk, FrankaErasmus University Medical Center (författare)
  • van den Hout-van Vroonhoven, Mirjam CgnErasmus University Medical Center (författare)
  • Ignatowicz, LechLund University,Lunds universitet,Dermatologi och venereologi, Lund,Sektion III,Institutionen för kliniska vetenskaper, Lund,Medicinska fakulteten,Dermatology and Venereology (Lund),Section III,Department of Clinical Sciences, Lund,Faculty of Medicine(Swepub:lu)med-lio (författare)
  • Catar, RusanCharité - University Medicine Berlin (författare)
  • Strini, TanjaErasmus University Medical Center (författare)
  • Korevaar, Sander SErasmus University Medical Center (författare)
  • van IJcken, Wilfred Fj (författare)
  • Betjes, Michiel GhErasmus University Medical Center (författare)
  • Franquesa, MarcellaErasmus University Medical Center (författare)
  • Moll, GuidoKarolinska Institutet (författare)
  • Baan, Carla CErasmus University Medical Center (författare)
  • Erasmus University Medical CenterDermatologi och venereologi, Lund (creator_code:org_t)

Sammanhörande titlar

  • Ingår i:Stem Cells and Development: Mary Ann Liebert Inc1557-85341547-3287

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