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- Plieva, FatimaLund University,Lunds universitet,Bioteknik,Centrum för tillämpade biovetenskaper,Kemiska institutionen,Institutioner vid LTH,Lunds Tekniska Högskola,Biotechnology,Center for Applied Life Sciences,Department of Chemistry,Departments at LTH,Faculty of Engineering, LTH (author)
Characterization of supermacroporous monolithic polyacrylamide based matrices designed for chromatography of bioparticles
- Article/chapterEnglish2004
Publisher, publication year, extent ...
- Elsevier BV,2004
Numbers
- LIBRIS-ID:oai:lup.lub.lu.se:dfb40dbf-46ae-4ada-9c3d-8da746b0c760
- https://lup.lub.lu.se/record/140665URI
- https://doi.org/10.1016/j.jchromb.2004.01.050DOI
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- Language:English
- Summary in:English
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- Supermacroporous monolithic acrylamide (AAm)-based cryogels were prepared by radical cryo-polymerizaton (polymerization in the moderately frozen system) of AAm with functional monomers and cross-linker N,N'-methylene-bis-acrylamide (MBAAm). Electron microscopy studies revealed supermacroporous structure of the developed cryogels with pore size of 5-100 mum. Cryogel porosity depended on cryo-polymerization conditions. More than 90% of the monolithic bed volume is the interconnected supermacropores filled with water and less than 10% of the monolithic volume is pore walls. The total protein binding capacity (lysozyme in the case of immobilized metal affinity chromatography (IMAC) column and bovine serum albumin (BSA) in the case of anion-exchange (AE) column) was independent of the flow rates till 600 cm/h. Chromatographic behavior of E. coli cells when a cell suspension was applied to ion-exchange cryogel columns depended on both the density of functional ligand and the porosity of the cryogel. (C) 2004 Elsevier B.V. All rights reserved.
Subject headings and genre
- TEKNIK OCH TEKNOLOGIER Industriell bioteknik hsv//swe
- ENGINEERING AND TECHNOLOGY Industrial Biotechnology hsv//eng
- Monolithic column
- Bioparticles
- Cryogel
- Supermacroporous polyacrylamide
Added entries (persons, corporate bodies, meetings, titles ...)
- Savina, IrinaLund University,Lunds universitet,Bioteknik,Centrum för tillämpade biovetenskaper,Kemiska institutionen,Institutioner vid LTH,Lunds Tekniska Högskola,Biotechnology,Center for Applied Life Sciences,Department of Chemistry,Departments at LTH,Faculty of Engineering, LTH(Swepub:lu)biot-is0 (author)
- Deraz, SaharLund University,Lunds universitet,Bioteknik,Centrum för tillämpade biovetenskaper,Kemiska institutionen,Institutioner vid LTH,Lunds Tekniska Högskola,Biotechnology,Center for Applied Life Sciences,Department of Chemistry,Departments at LTH,Faculty of Engineering, LTH(Swepub:lu)biot-sd8 (author)
- Andersson, JonatanLund University,Lunds universitet,Bioteknik,Centrum för tillämpade biovetenskaper,Kemiska institutionen,Institutioner vid LTH,Lunds Tekniska Högskola,Biotechnology,Center for Applied Life Sciences,Department of Chemistry,Departments at LTH,Faculty of Engineering, LTH(Swepub:lu)biot-ja8 (author)
- Galaev, IgorLund University,Lunds universitet,Bioteknik,Centrum för tillämpade biovetenskaper,Kemiska institutionen,Institutioner vid LTH,Lunds Tekniska Högskola,Biotechnology,Center for Applied Life Sciences,Department of Chemistry,Departments at LTH,Faculty of Engineering, LTH(Swepub:lu)biot-iga (author)
- Mattiasson, BoLund University,Lunds universitet,Bioteknik,Centrum för tillämpade biovetenskaper,Kemiska institutionen,Institutioner vid LTH,Lunds Tekniska Högskola,Biotechnology,Center for Applied Life Sciences,Department of Chemistry,Departments at LTH,Faculty of Engineering, LTH(Swepub:lu)biot-bma (author)
- BioteknikCentrum för tillämpade biovetenskaper (creator_code:org_t)
Related titles
- In:Journal of Chromatography. B: Elsevier BV807:1, s. 129-1371873-376X1570-0232
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