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The interaction of ...
The interaction of styrene maleic acid copolymers with phospholipids in Langmuir monolayers, vesicles and nanodiscs; a structural study
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- Hall, Stephen C.L. (författare)
- Diamond Light Source,University of Birmingham,Rutherford Appleton Laboratory
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- Tognoloni, Cecilia (författare)
- University of Bath
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- Campbell, Richard A. (författare)
- Institut Laue Langevin,University of Manchester
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- Richens, Joanna (författare)
- University of Nottingham
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- O'Shea, Paul (författare)
- Lancaster University,University of Nottingham
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- Terry, Ann E. (författare)
- Lund University,Lunds universitet,MAX IV-laboratoriet,MAX IV Laboratory
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- Price, Gareth J. (författare)
- University of Bath
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- Dafforn, Tim R. (författare)
- University of Birmingham
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- Edler, Karen J. (författare)
- Lund University,Lunds universitet,Kemiska institutionen,Institutioner vid LTH,Lunds Tekniska Högskola,Department of Chemistry,Departments at LTH,Faculty of Engineering, LTH,University of Bath
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- Arnold, Thomas (författare)
- Diamond Light Source,University of Bath,European Spallation Source ERIC, Sweden,Rutherford Appleton Laboratory
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(creator_code:org_t)
- Elsevier BV, 2022
- 2022
- Engelska 17 s.
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Ingår i: Journal of Colloid and Interface Science. - : Elsevier BV. - 0021-9797. ; 625, s. 220-236
- Relaterad länk:
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http://dx.doi.org/10... (free)
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https://lup.lub.lu.s...
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https://doi.org/10.1...
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Abstract
Ämnesord
Stäng
- Hypothesis: Self-assembly of amphipathic styrene maleic acid copolymers with phospholipids in aqueous solution results in the formation of ‘nanodiscs’ containing a planar segment of phospholipid bilayer encapsulated by a polymer belt. Recently, studies have reported that lipids rapidly exchange between both nanodiscs in solution and external sources of lipids. Outstanding questions remain regarding details of polymer-lipid interactions, factors influencing lipid exchange and structural effects of such exchange processes. Here, the dynamic behaviour of nanodiscs is investigated, specifically the role of membrane charge and polymer chemistry. Experiments: Two model systems are investigated: fluorescently labelled phospholipid vesicles, and Langmuir monolayers of phospholipids. Using fluorescence spectroscopy and time-resolved neutron reflectometry, the membrane potential, monolayer structure and composition are monitored with respect to time upon polymer and nanodisc interactions. Findings: In the presence of external lipids, polymer chains embed throughout lipid membranes, the extent of which is governed by the net membrane charge. Nanodiscs stabilised by three different polymers will all exchange lipids and polymer with monolayers to differing extents, related to the properties of the stabilising polymer belt. These results demonstrate the dynamic nature of nanodiscs which interact with the local environment and are likely to deposit both lipids and polymer at all stages of use.
Ämnesord
- NATURVETENSKAP -- Kemi -- Fysikalisk kemi (hsv//swe)
- NATURAL SCIENCES -- Chemical Sciences -- Physical Chemistry (hsv//eng)
Nyckelord
- Adsorption
- Langmuir monolayer
- Lipid Exchange
- Nanodisc
- Neutron reflectometry
- Polymer
- Reversible, Addition−Fragmentation Chain-Transfer (RAFT)
- Styrene Maleic Acid (SMA)
- Styrene Maleic Acid Lipid Particle (SMALP)
Publikations- och innehållstyp
- art (ämneskategori)
- ref (ämneskategori)
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Till lärosätets databas
- Av författaren/redakt...
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Hall, Stephen C. ...
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Tognoloni, Cecil ...
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Campbell, Richar ...
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Richens, Joanna
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O'Shea, Paul
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Terry, Ann E.
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visa fler...
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Price, Gareth J.
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Dafforn, Tim R.
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Edler, Karen J.
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Arnold, Thomas
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visa färre...
- Om ämnet
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- NATURVETENSKAP
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NATURVETENSKAP
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och Kemi
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och Fysikalisk kemi
- Artiklar i publikationen
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Journal of Collo ...
- Av lärosätet
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Lunds universitet