Multi-Functional Desaturases in Two Spodoptera Moths with ∆11 and ∆12 Desaturation Activities

Xia, Yi HanLund University,Lunds universitet,Funktionell zoologi,Biologiska institutionen,Naturvetenskapliga fakulteten,Functional zoology,Department of Biology,Faculty of Science (författare)

The beet armyworm, Spodoptera exigua, uses (Z,E)-9,12-tetradecadienyl acetate as the major component of its sex pheromone. Previous isotope-labeling experiments demonstrated an unusual ∆12 desaturase activity involved in the biosynthesis of this compound; however, the putative ∆12 desaturase gene has not been reported to date. In the present study, we confirmed this ∆12 desaturation pathway by in vivo labeling experiments, and characterized candidate desaturase genes in a yeast heterologous expression system. We demonstrated that a pheromone gland-specific desaturase, SexiDes5, uses palmitic acid and the subsequently chain-shortened product (Z)-9-tetradecenoic acid as substrates to produce (Z)-11-hexadecenoic and (Z,E)-9,12-tetradecadienoic acids, respectively. In addition, the homologous desaturase SlitDes5 from the congeneric Spodoptera litura had analogous functions.

Zhang, Ya NanHuaibei Normal University (författare)
Ding, Bao JianLund University,Lunds universitet,Funktionell zoologi,Biologiska institutionen,Naturvetenskapliga fakulteten,Functional zoology,Department of Biology,Faculty of Science(Swepub:lu)ekol-bod (författare)
Wang, Hong LeiLund University,Lunds universitet,Funktionell zoologi,Biologiska institutionen,Naturvetenskapliga fakulteten,Functional zoology,Department of Biology,Faculty of Science(Swepub:lu)ekol-hlw (författare)
Löfstedt, ChristerLund University,Lunds universitet,Funktionell zoologi,Biologiska institutionen,Naturvetenskapliga fakulteten,Functional zoology,Department of Biology,Faculty of Science(Swepub:lu)ekol-clo (författare)
Xia, Yihan,1990Lunds universitet,Lund University(Swepub:cth)yihanx (författare)
Ding, BaojianLunds universitet,Lund University (författare)
Wang, HongleiLunds universitet,Lund University (författare)
Funktionell zoologiBiologiska institutionen (creator_code:org_t)

Ingår i:Journal of Chemical Ecology: Springer Science and Business Media LLC45:4, s. 378-3870098-03311573-1561

Av författaren/redakt...: Xia, Yi Han; Zhang, Ya Nan; Ding, Bao Jian; Wang, Hong Lei; Löfstedt, Christ ...; Xia, Yihan, 1990; visa fler...; Ding, Baojian; Wang, Honglei; visa färre...

Om ämnet

Kungliga biblioteket hanterar dina personuppgifter i enlighet med EU:s dataskyddsförordning (2018), GDPR. Läs mer om hur det funkar här.
Så här hanterar KB dina uppgifter vid användning av denna tjänst.