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In Vivo and In Vitr...
In Vivo and In Vitro Pro-Fibrotic Response of Lung-Resident Mesenchymal Stem Cells from Patients with Idiopathic Pulmonary Fibrosis
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- Escarrer-Garau, Gabriel (author)
- University of the Balearic Islands
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- Martín-Medina, Aina (author)
- Health Research Institute of the Balearic Islands (IdISBa)
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- Truyols-Vives, Joan (author)
- University of the Balearic Islands
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- Gómez-Bellvert, Cristina (author)
- Hospital Universitario Son Espases
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- Elowsson, Linda (author)
- Lund University,Lunds universitet,Lungbiologi,Forskargrupper vid Lunds universitet,Lung Biology,Lund University Research Groups
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- Westergren-Thorsson, Gunilla (author)
- Lund University,Lunds universitet,Lungbiologi,Forskargrupper vid Lunds universitet,Lund University Bioimaging Center,Medicinska fakulteten,WCMM- Wallenberg center för molekylär medicinsk forskning,Lung Biology,Lund University Research Groups,Faculty of Medicine,WCMM-Wallenberg Centre for Molecular Medicine
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- Molina-Molina, Maria (author)
- CIBER Enfermedades Respiratorias (CIBERES)
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- Mercader-Barceló, Josep (author)
- University of the Balearic Islands
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- Sala-Llinàs, Ernest (author)
- CIBER Enfermedades Respiratorias (CIBERES)
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(creator_code:org_t)
- 2024
- 2024
- English.
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In: Cells. - 2073-4409. ; 13:2, s. 1-13
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http://dx.doi.org/10... (free)
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https://lup.lub.lu.s...
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https://doi.org/10.3...
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Abstract
Subject headings
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- Lung-resident mesenchymal stem cells (LR-MSC) are thought to participate in idiopathic pulmonary fibrosis (IPF) by differentiating into myofibroblasts. On the other hand, LR-MSC in IPF patients present senescence-related features. It is unclear how they respond to a profibrotic environment. Here, we investigated the profibrotic response of LR-MSC isolated from IPF and control (CON) patients. LR-MSC were inoculated in mice 48 h after bleomycin (BLM) instillation to analyze their contribution to lung damage. In vitro, LR-MSC were exposed to TGFβ. Mice inoculated with IPF LR-MSC exhibited worse maintenance of their body weight. The instillation of either IPF or CON LR-MSC sustained BLM-induced histological lung damage, bronchoalveolar lavage fluid cell count, and the expression of the myofibroblast marker, extracellular matrix (ECM) proteins, and proinflammatory cytokines in the lungs. In vitro, IPF LR-MSC displayed higher basal protein levels of aSMA and fibronectin than CON LR-MSC. However, the TGFβ response in the expression of TGFβ, aSMA, and ECM genes was attenuated in IPF LR-MSC. In conclusion, IPF LR-MSC have acquired myofibroblastic features, but their capacity to further respond to profibrotic stimuli seems to be attenuated. In an advanced stage of the disease, LR-MSC may participate in disease progression owing to their limited ability to repair epithelial damage.
Subject headings
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Cell- och molekylärbiologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Cell and Molecular Biology (hsv//eng)
Keyword
- Humans
- Animals
- Mice
- Idiopathic Pulmonary Fibrosis
- Bronchoalveolar Lavage Fluid
- Bleomycin
- Extracellular Matrix Proteins
- Lung
- Transforming Growth Factor beta
Publication and Content Type
- art (subject category)
- ref (subject category)
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