BtuB-dependent infection of the T5-like Yersinia phage φr2-01

• Yersinia enterocolitica is a food-borne Gram-negative pathogen responsible for several gastrointestinal disorders. Host-specific lytic bacteriophages have been increasingly used recently as an alternative or complementary treatment to combat bacterial infections, especially when antibiotics fail. Here, we describe the proteogenomic characterization and host receptor identification of the siphovirus vB_YenS_φR2-01 (in short, φR2-01) that infects strains of several Yersinia enterocolitica serotypes. The φR2-01 genome contains 154 predicted genes, 117 of which encode products that are homologous to those of Escherichia bacteriophage T5. The φR2-01 and T5 genomes are largely syntenic, with the major differences residing in areas encoding hypothetical φR2-01 proteins. Label-free massspectrometry-based proteomics confirmed the expression of 90 of the φR2-01 genes, with 88 of these being either phage particle structural or phage-particle-associated proteins. In vitro transposon-based host mutagenesis and φR2-01 adsorption experiments identified the outer membrane vitamin B12 receptor BtuB as the host receptor. This study provides a proteogenomic characterization of a T5-type bacteriophage and identifies specific Y. enterocolitica strains sensitive to infection with possible future applications of φR2-01 as a food biocontrol or phage therapy agent.

Ämnesord

MEDICIN OCH HÄLSOVETENSKAP  -- Medicinska och farmaceutiska grundvetenskaper -- Mikrobiologi inom det medicinska området (hsv//swe)

MEDICAL AND HEALTH SCIENCES  -- Basic Medicine -- Microbiology in the medical area (hsv//eng)

NATURVETENSKAP  -- Biologi -- Mikrobiologi (hsv//swe)

NATURAL SCIENCES  -- Biological Sciences -- Microbiology (hsv//eng)

Nyckelord

Bacteriophage

BtuB

Escherichia phage T5

Genome

Phage φR2-01

Proteome

Yersinia enterocolitica

Publikations- och innehållstyp

art (ämneskategori)

ref (ämneskategori)