Bioinformatic Analysis of the Wound Peptidome Reveals Potential Biomarkers and Antimicrobial Peptides

↓ Direkt till sidans innehåll
↓ Direkt till sidans sekundära innehåll (sidomenyn)

Sökning: WFRF:(Schmidtchen A.) > Bioinformatic Analy...

Hartman, ErikSkåne University Hospital (författare)

Bioinformatic Analysis of the Wound Peptidome Reveals Potential Biomarkers and Antimicrobial Peptides

Artikel/kapitelEngelska2021

Förlag, utgivningsår, omfång ...

2021-02-03
Frontiers Media SA,2021

Nummerbeteckningar

LIBRIS-ID:oai:lup.lub.lu.se:ea5ea26c-8883-4262-a2cb-7c29c0f4348f
https://lup.lub.lu.se/record/ea5ea26c-8883-4262-a2cb-7c29c0f4348fURI
https://doi.org/10.3389/fimmu.2020.620707DOI

Kompletterande språkuppgifter

Språk:engelska
Sammanfattning på:engelska

Ingår i deldatabas

SwePubSwePub

Klassifikation

Ämneskategori:art swepub-publicationtype
Ämneskategori:ref swepub-contenttype

Anmärkningar

Wound infection is a common and serious medical condition with an unmet need for improved diagnostic tools. A peptidomic approach, aided by mass spectrometry and bioinformatics, could provide novel means of identifying new peptide biomarkers for wound healing and infection assessment. Wound fluid is suitable for peptidomic analysis since it is both intimately tied to the wound environment and is readily available. In this study we investigate the peptidomes of wound fluids derived from surgical drainages following mastectomy and from wound dressings following facial skin grafting. By applying sorting algorithms and open source third party software to peptidomic label free tandem mass spectrometry data we provide an unbiased general methodology for analyzing and differentiating between peptidomes. We show that the wound fluid peptidomes of patients are highly individualized. However, differences emerge when grouping the patients depending on wound type. Furthermore, the abundance of peptides originating from documented antimicrobial regions of hemoglobin in infected wounds may contribute to an antimicrobial wound environment, as determined by in silico analysis. We validate our findings by compiling literature on peptide biomarkers and peptides of physiological significance and cross checking the results against our dataset, demonstrating that well-documented peptides of immunological significance are abundant in infected wounds, and originate from certain distinct regions in proteins such as hemoglobin and fibrinogen. Ultimately, we have demonstrated the power using sorting algorithms and open source software to help yield insights and visualize peptidomic data.

Ämnesord och genrebeteckningar

MEDICIN OCH HÄLSOVETENSKAP Klinisk medicin Dermatologi och venereologi hsv//swe
MEDICAL AND HEALTH SCIENCES Clinical Medicine Dermatology and Venereal Diseases hsv//eng
MEDICIN OCH HÄLSOVETENSKAP Klinisk medicin Infektionsmedicin hsv//swe
MEDICAL AND HEALTH SCIENCES Clinical Medicine Infectious Medicine hsv//eng
antimicrobial peptide
bioinformatics
biomarkers
hemoglobin
mass spectrometry
peptidomics
wound healing
wound infection

Biuppslag (personer, institutioner, konferenser, titlar ...)

Wallblom, KarlLund University,Lunds universitet,Dermatologi och venereologi, Lund,Sektion III,Institutionen för kliniska vetenskaper, Lund,Medicinska fakulteten,Dermatology and Venereology (Lund),Section III,Department of Clinical Sciences, Lund,Faculty of Medicine(Swepub:lu)ka0580wa (författare)
van der Plas, Mariena J.A.Lund University,Lunds universitet,Dermatologi och venereologi, Lund,Sektion III,Institutionen för kliniska vetenskaper, Lund,Medicinska fakulteten,Schmidtchen lab,Forskargrupper vid Lunds universitet,Dermatology and Venereology (Lund),Section III,Department of Clinical Sciences, Lund,Faculty of Medicine,Schmidtchen Lab,Lund University Research Groups,University of Copenhagen(Swepub:lu)med-mja (författare)
Petrlova, JitkaLund University,Lunds universitet,Dermatologi och venereologi, Lund,Sektion III,Institutionen för kliniska vetenskaper, Lund,Medicinska fakulteten,Schmidtchen lab,Forskargrupper vid Lunds universitet,Dermatology and Venereology (Lund),Section III,Department of Clinical Sciences, Lund,Faculty of Medicine,Schmidtchen Lab,Lund University Research Groups(Swepub:lu)med-jkp (författare)
Cai, JunUniversity of Copenhagen (författare)
Saleh, KarimLund University,Lunds universitet,Dermatologi och venereologi, Lund,Sektion III,Institutionen för kliniska vetenskaper, Lund,Medicinska fakulteten,Schmidtchen lab,Forskargrupper vid Lunds universitet,Dermatology and Venereology (Lund),Section III,Department of Clinical Sciences, Lund,Faculty of Medicine,Schmidtchen Lab,Lund University Research Groups,Skåne University Hospital(Swepub:lu)med-ksa (författare)
Kjellström, SvenLund University,Lunds universitet,Masspektrometri,Sektion V,Institutionen för kliniska vetenskaper, Lund,Medicinska fakulteten,BioMS,Forskargrupper vid Lunds universitet,Mass Spectrometry,Section V,Department of Clinical Sciences, Lund,Faculty of Medicine,Lund University Research Groups(Swepub:lu)bioc-skj (författare)
Schmidtchen, ArturLund University,Lunds universitet,Dermatologi och venereologi, Lund,Sektion III,Institutionen för kliniska vetenskaper, Lund,Medicinska fakulteten,Dermatology and Venereology (Lund),Section III,Department of Clinical Sciences, Lund,Faculty of Medicine,Skåne University Hospital,University of Copenhagen(Swepub:lu)derm-asc (författare)
Skåne University HospitalDermatologi och venereologi, Lund (creator_code:org_t)

Sammanhörande titlar

Ingår i:Frontiers in Immunology: Frontiers Media SA111664-3224

Internetlänk

Hitta via bibliotek

Frontiers in Immunology (Sök värdpublikationen i LIBRIS)

Till lärosätets databas

Find more in SwePub

By the author/editor: Hartman, Erik; Wallblom, Karl; van der Plas, Ma ...; Petrlova, Jitka; Cai, Jun; Saleh, Karim; show more...; Kjellström, Sven; Schmidtchen, Art ...; show less...

About the subject

MEDICIN OCH HÄLSOVETENSKAP: MEDICIN OCH HÄLS ...; and Klinisk medicin; and Dermatologi och ...

MEDICIN OCH HÄLSOVETENSKAP: MEDICIN OCH HÄLS ...; and Klinisk medicin; and Infektionsmedici ...

Articles in the publication: Frontiers in Imm ...

By the university: Lund University

Search outside SwePub

Extend your search to:: Google; Google Book Search; Google Scholar

Kungliga biblioteket hanterar dina personuppgifter i enlighet med EU:s dataskyddsförordning (2018), GDPR. Läs mer om hur det funkar här.
Så här hanterar KB dina uppgifter vid användning av denna tjänst.

LIBRIS.kb.se