onr:"swepub:oai:lup.lub.lu.se:ea83713e-eaf9-4cef-b156-8dccaa415c65"
Search: onr:"swepub:oai:lup.lub.lu.se:ea83713e-eaf9-4cef-b156-8dccaa415c65" > Functional activity...
- 1 of 1
- Previous record
- Next record
- To hitlist
- Leanza, GLund University,Lunds universitet,Basala gangliernas patofysiologi,Forskargrupper vid Lunds universitet,Basal Ganglia Pathophysiology,Lund University Research Groups (author)
Functional activity of intrahippocampal septal grafts is regulated by catecholaminergic host afferents as studied by microdialysis of acetylcholine
- Article/chapterEnglish1993
Publisher, publication year, extent ...
- Elsevier BV,1993
Numbers
- LIBRIS-ID:oai:lup.lub.lu.se:ea83713e-eaf9-4cef-b156-8dccaa415c65
- https://lup.lub.lu.se/record/ea83713e-eaf9-4cef-b156-8dccaa415c65URI
- https://doi.org/10.1016/0006-8993(93)90427-oDOI
Supplementary language notes
- Language:English
- Summary in:English
Part of subdatabase
- SwePubSwePub
Classification
Notes
- Previous microdialysis experiments have shown that acetylcholine (ACh) release from septal grafts in the hippocampus of awake rats is influenced by the behaviour of the animals, which strongly suggests that the host brain can exert a regulatory control over the activity of the grafted neurons. Since the activity of the normal septo-hippocampal cholinergic system is likely to be regulated, in part, by brainstem catecholaminergic afferents, we wished to study the effect of catecholaminergic drugs on ACh release in the hippocampus reinnervated by septal grafts. Rats were subjected to a unilateral aspirative fimbria-fornix (FF) transection and grafted with tissue from the fetal septal-diagonal band area, either as a cell suspension injection into the depth of the hippocampus or as a solid implant in the FF lesion cavity. Microdialysis of ACh release was carried out 17-20 months after transplantation in awake, freely-moving animals. The reduction in steady-state ACh overflow induced by the FF lesion (-81%) was restored to normal or above normal levels in rats with either solid or suspension grafts. In normal rats, systemic administration of apomorphine (2.0 mg/kg, s.c.) or amphetamine (2.5 mg/kg, i.p.) caused a 3.7 (+189%) or 7.8 (+301%) pmol/15 min increase in ACh overflow compared to the previous baseline level, respectively. The drug-induced increases in ACh levels in the FF-lesioned controls was substantially lower than normal (86-89% reduction). Both apomorphine and amphetamine resulted in an approximately two-fold increase in hippocampal ACh release in rats with suspension grafts. These responses were significantly increased over those seen in rats with FF lesions only, but they tended to be lower and more variable than normal. Rats with solid septal grafts responded significantly stronger than FF lesion controls to amphetamine with two-fold increased ACh overflow, whereas the response to apomorphine was less clear-cut. Pretreatment with the catecholamine synthesis blocker alpha-methyl-p-tyrosine (AMPT; 200 mg/kg x 3) did not affect steady-state or apomorphine-stimulated release of ACh in any of the groups, whereas the effect of amphetamine was abolished in both normal and grafted rats. The results suggest that ACh release derived from septal grafts in the hippocampus, similar to the normal septo-hippocampal system, can be affected by manipulations of the host catecholaminergic systems. This mechanism may, at least in part, underlie the ability of the host brain to influence and control the activity of grafted cholinergic neurons.
Subject headings and genre
- MEDICIN OCH HÄLSOVETENSKAP Medicinska och farmaceutiska grundvetenskaper Neurovetenskaper hsv//swe
- MEDICAL AND HEALTH SCIENCES Basic Medicine Neurosciences hsv//eng
- Acetylcholine/administration & dosage
- Afferent Pathways/drug effects
- Amphetamine/pharmacology
- Animals
- Apomorphine/pharmacology
- Catecholamines/metabolism
- Female
- Fetal Tissue Transplantation
- Hippocampus
- Methyltyrosines/pharmacology
- Microdialysis
- Rats
- Rats, Sprague-Dawley
- Receptors, Dopamine/drug effects
- Septum Pellucidum/transplantation
- Transplantation, Heterotopic
- Tyrosine 3-Monooxygenase/antagonists & inhibitors
- alpha-Methyltyrosine
Added entries (persons, corporate bodies, meetings, titles ...)
- Nilsson, O GLund University,Lunds universitet,Neurokirurgi,Sektion IV,Institutionen för kliniska vetenskaper, Lund,Medicinska fakulteten,Neurosurgery,Section IV,Department of Clinical Sciences, Lund,Faculty of Medicine(Swepub:lu)nkir-oni (author)
- Björklund, ALund University,Lunds universitet,Neurobiologi,Forskargrupper vid Lunds universitet,Neurobiology,Lund University Research Groups(Swepub:lu)mphy-abj (author)
- Basala gangliernas patofysiologiForskargrupper vid Lunds universitet (creator_code:org_t)
Related titles
- In:Brain Research: Elsevier BV618:1, s. 47-560006-8993
Internet link
Find in a library
- Brain Research (Search for host publication in LIBRIS)
To the university's database
- 1 of 1
- Previous record
- Next record
- To hitlist
Find more in SwePub
- By the author/editor
- Leanza, G
- Nilsson, O G
- Björklund, A
- About the subject
-
- MEDICAL AND HEALTH SCIENCES
- MEDICAL AND HEAL ...
- and Basic Medicine
- and Neurosciences
- Articles in the publication
- Brain Research
- By the university
- Lund University
Search outside SwePub
- Extend your search to:
- Google Book Search
- Google Scholar
Kungliga biblioteket hanterar dina personuppgifter i enlighet med EU:s dataskyddsförordning (2018), GDPR. Läs mer om hur det funkar här.
Så här hanterar KB dina uppgifter vid användning av denna tjänst.
- Copyright © LIBRIS - National Library Systems
- LIBRIS.kb.se
