Search: id:"swepub:oai:lup.lub.lu.se:ee5f0b95-b933-4553-bc6e-0b8dbff1644c" >
Reference standards...
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Malapelle, UmbertoUniversity of Naples Federico II
(author)
Reference standards for gene fusion molecular assays on cytological samples : an international validation study
- Article/chapterEnglish2023
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LIBRIS-ID:oai:lup.lub.lu.se:ee5f0b95-b933-4553-bc6e-0b8dbff1644c
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https://lup.lub.lu.se/record/ee5f0b95-b933-4553-bc6e-0b8dbff1644cURI
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https://doi.org/10.1136/jclinpath-2021-207825DOI
Supplementary language notes
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Language:English
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Summary in:English
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Subject category:art swepub-publicationtype
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Subject category:ref swepub-contenttype
Notes
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Aims Gene fusions assays are key for personalised treatments of advanced human cancers. Their implementation on cytological material requires a preliminary validation that may make use of cell line slides mimicking cytological samples. In this international multi-institutional study, gene fusion reference standards were developed and validated. Methods Cell lines harbouring EML4(13)–ALK(20) and SLC34A2(4)–ROS1(32) gene fusions were adopted to prepare reference standards. Eight laboratories (five adopting amplicon-based and three hybridisation-based platforms) received, at different dilution points two sets of slides (slide A 50.0%, slide B 25.0%, slide C 12.5% and slide D wild type) stained by Papanicolaou (Pap) and May Grunwald Giemsa (MGG). Analysis was carried out on a total of 64 slides. Results Four (50.0%) out of eight laboratories reported results on all slides and dilution points. While 12 (37.5%) out of 32 MGG slides were inadequate, 27 (84.4%) out of 32 Pap slides produced libraries adequate for variant calling. The laboratories using hybridisation-based platforms showed the highest rate of inadequate results (13/24 slides, 54.2%). Conversely, only 10.0% (4/40 slides) of inadequate results were reported by laboratories adopting amplicon-based platforms. Conclusions Reference standards in cytological format yield better results when Pap staining and processed by amplicon-based assays. Further investigation is required to optimise these standards for MGG stained cells and for hybridisation-based approaches.
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Added entries (persons, corporate bodies, meetings, titles ...)
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Pepe, FrancescoUniversity of Naples Federico II
(author)
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Pisapia, PasqualeUniversity of Naples Federico II
(author)
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Altimari, AnnalisaSt. Orsola-Malpighi University Hospital
(author)
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Bellevicine, ClaudioUniversity of Naples Federico II
(author)
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Brunnström, HansLund University,Lunds universitet,Förbättrad diagnostik och prognostik vid lungcancer och metastaser till lunga,Forskargrupper vid Lunds universitet,LUCC: Lunds universitets cancercentrum,Övriga starka forskningsmiljöer,Patologi, Lund,Sektion V,Institutionen för kliniska vetenskaper, Lund,Medicinska fakulteten,Improved diagnostics and prognostics of lung cancer and metastases to the lungs,Lund University Research Groups,LUCC: Lund University Cancer Centre,Other Strong Research Environments,Pathology, Lund,Section V,Department of Clinical Sciences, Lund,Faculty of Medicine(Swepub:lu)med-hsb
(author)
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Bruno, RossellaUniversity of Pisa
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Büttner, ReinhardUniversity of Cologne
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Cirnes, LuisUniversity of Porto
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De Andrea, Carlos E.University of Navarra
(author)
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de Biase, DarioUniversity of Bologna
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Dumur, Catherine I.Aurora Diagnostics
(author)
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Lindquist, Kajsa EricsonLund University,Lunds universitet,Patologi, Lund,Sektion V,Institutionen för kliniska vetenskaper, Lund,Medicinska fakulteten,Pathology, Lund,Section V,Department of Clinical Sciences, Lund,Faculty of Medicine(Swepub:lu)onk-ker
(author)
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Fontanini, GabriellaUniversity of Pisa
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Gautiero, EugenioUniversity of Milano-Bicocca
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Gentien, DavidCurie Institute, Paris
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Hofman, PaulCHU Hôpital Pasteur 2, Nice
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Hofman, VeroniqueCHU Hôpital Pasteur 2, Nice
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Iaccarino, AntoninoUniversity of Naples Federico II
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Lozano, Maria DoloresUniversity of Navarra
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Mayo-De-Las-Casas, ClaraUniversity Hospital Dexeus
(author)
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Merkelbach-Bruse, SabineUniversity of Cologne
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Pagni, FabioUniversity of Milano-Bicocca
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Roman, RuthUniversity Hospital Dexeus
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Schmitt, Fernando C.University of Porto
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Siemanowski, JannaUniversity of Cologne
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Roy-Chowdhuri, SinchitaUniversity of Texas
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Tallini, GiovanniUniversity of Bologna
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Tresserra, FrancescUniversity Hospital Dexeus
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Borght, Sara VanderCatholic University of Leuven
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Vielh, PhilippeThe American Hospital of Paris
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Vigliar, ElenaUniversity of Naples Federico II
(author)
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Vita, Giulia Anna CarmenIRCCS-CROB, Referral Cancer Center of Basilicata
(author)
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Weynand, BirgitCatholic University of Leuven
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Rosell, RafaelHospital Municipal de Badalona
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Vila, Miguel Angel MolinaUniversity Hospital Dexeus
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Troncone, GiancarloUniversity of Naples Federico II
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University of Naples Federico IISt. Orsola-Malpighi University Hospital
(creator_code:org_t)
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In:Journal of Clinical Pathology: BMJ76:1, s. 47-520021-97461472-4146
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Pepe, Francesco
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Pisapia, Pasqual ...
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Altimari, Annali ...
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Bellevicine, Cla ...
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Brunnström, Hans
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Bruno, Rossella
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Büttner, Reinhar ...
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Cirnes, Luis
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de Biase, Dario
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Dumur, Catherine ...
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Lindquist, Kajsa ...
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Fontanini, Gabri ...
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Gautiero, Eugeni ...
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Gentien, David
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Hofman, Paul
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Hofman, Veroniqu ...
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Iaccarino, Anton ...
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Lozano, Maria Do ...
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Mayo-De-Las-Casa ...
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Schmitt, Fernand ...
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Roy-Chowdhuri, S ...
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Tallini, Giovann ...
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Tresserra, Franc ...
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Vielh, Philippe
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Vigliar, Elena
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