Adipose cell size changes are associated with a drastic actin remodeling

Hansson, BjörnLund University,Lunds universitet,Glukostransport och proteintrafficking,Forskargrupper vid Lunds universitet,Glucose Transport and Protein Trafficking,Lund University Research Groups (författare)

Adipose tissue plays a major role in regulating whole-body insulin sensitivity and energy metabolism. To accommodate surplus energy, the tissue rapidly expands by increasing adipose cell size (hypertrophy) and cell number (hyperplasia). Previous studies have shown that enlarged, hypertrophic adipocytes are less responsive to insulin, and that adipocyte size could serve as a predictor for the development of type 2 diabetes. In the present study, we demonstrate that changes in adipocyte size correlate with a drastic remodeling of the actin cytoskeleton. Expansion of primary adipocytes following 2 weeks of high-fat diet (HFD)-feeding in C57BL6/J mice was associated with a drastic increase in filamentous (F)-actin as assessed by fluorescence microscopy, increased Rho-kinase activity, and changed expression of actin-regulating proteins, favoring actin polymerization. At the same time, increased cell size was associated with impaired insulin response, while the interaction between the cytoskeletal scaffolding protein IQGAP1 and insulin receptor substrate (IRS)-1 remained intact. Reversed feeding from HFD to chow restored cell size, insulin response, expression of actin-regulatory proteins and decreased the amount of F-actin filaments. Together, we report a drastic cytoskeletal remodeling during adipocyte expansion, a process which could contribute to deteriorating adipocyte function.

Morén, BjörnLund University,Lunds universitet,Glukostransport och proteintrafficking,Forskargrupper vid Lunds universitet,Glucose Transport and Protein Trafficking,Lund University Research Groups(Swepub:lu)bj6337mo (författare)
Fryklund, ClaesLund University,Lunds universitet,Glukostransport och proteintrafficking,Forskargrupper vid Lunds universitet,Glucose Transport and Protein Trafficking,Lund University Research Groups(Swepub:lu)cl0752fr (författare)
Vliex, LarsLund University,Maastricht University (författare)
Wasserstrom, SebastianLund University,Lunds universitet,Glukostransport och proteintrafficking,Forskargrupper vid Lunds universitet,Glucose Transport and Protein Trafficking,Lund University Research Groups(Swepub:lu)cob-saw (författare)
Albinsson, SebastianLund University,Lunds universitet,Molekylär kärlfysiologi,Forskargrupper vid Lunds universitet,Molecular Vascular Physiology,Lund University Research Groups(Swepub:lu)mphy-sal (författare)
Berger, KarinLund University,Lunds universitet,Molekylär nutrition,Forskargrupper vid Lunds universitet,Molecular Nutrition,Lund University Research Groups(Swepub:lu)medk-kbe (författare)
Stenkula, Karin G.Lund University,Lunds universitet,Glukostransport och proteintrafficking,Forskargrupper vid Lunds universitet,Glucose Transport and Protein Trafficking,Lund University Research Groups(Swepub:lu)med-ksu (författare)
Glukostransport och proteintraffickingForskargrupper vid Lunds universitet (creator_code:org_t)

Av författaren/redakt...: Hansson, Björn; Morén, Björn; Fryklund, Claes; Vliex, Lars; Wasserstrom, Seb ...; Albinsson, Sebas ...; visa fler...; Berger, Karin; Stenkula, Karin ...; visa färre...

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MEDICIN OCH HÄLSOVETENSKAP: MEDICIN OCH HÄLS ...; och Medicinska och f ...; och Cell och molekyl ...

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