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Heterozygous PINK1 ...
Heterozygous PINK1 p.G411S mutation increases risk for Parkinson's disease (PD)
- Artikel/kapitelEngelska2016
Förlag, utgivningsår, omfång ...
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2016-06-19
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Wiley,2016
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1 s.
Nummerbeteckningar
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LIBRIS-ID:oai:lup.lub.lu.se:f167e4d5-95bb-4e32-aef0-c7f2b23b6eaa
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https://lup.lub.lu.se/record/f167e4d5-95bb-4e32-aef0-c7f2b23b6eaaURI
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https://doi.org/10.1002/mds.26688DOI
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Språk:engelska
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Sammanfattning på:engelska
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Klassifikation
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Ämneskategori:kon swepub-publicationtype
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Ämneskategori:ref swepub-contenttype
Anmärkningar
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Objective: To investigate the possible disease-association and pathogenic mechanisms of heterozygous PINK1 mutations from a genetic, functional, and structural perspective. Background: It has been postulated that heterozygous mutations in recessive PD genes may increase disease risk. In particular, the PINK1 p.G411S mutation has been reported in families with dominant inheritance patterns, suggesting that it might confer a sizeable disease risk. Methods: We performed a pedigree analysis of seven patients with a heterozygous PINK1 p.G411S mutation with at least one additional affected family member. We screened five case-control series and performed a meta-analysis of previous studies that had examined the variant. For functional cell-based analyses, we used patients skin fibroblast from PINK1 p.G411S or p.Q456X heterozygotes and investigated endogenous protein levels and kinase activity by biochemistry and imaging. For structural analyses, we performed molecular modeling and generated monomeric and dimeric forms of wild type (WT) and mutant PINK1 protein. Using molecular dynamics simulations, we analyzed effects of the p.G411S mutation on WT PINK1 in a heterodimeric complex over time. Results: Our analyses revealed a genetic association of heterozygous PINK1 p.G411S mutation with an increased risk for PD and a possible dominant inheritance with incomplete co-segregation. In patients skin fibroblasts, we establish a dominant negative mode for heterozygous p.G411S mutations under endogenous conditions. While total PINK1 protein levels were similar to controls upon mitochondrial stress, cellular PINK1 kinase activity was significantly reduced in p.G411S heterozygotes compared to WT and importantly to p.Q456X heterozygotes, which resulted in 50% reduction of PINK1 protein levels. Structural analyses supported our hypothesis that the p.G411S mutation can poison PINK1 WT in a heterodimeric complex and thus effectively reduce cellular PINK1 kinase activity. This in turn impairs the protective functions of the PINK1/PARKIN-mediated mitochondrial quality control. Conclusions: Our study uncovers increased disease risk and molecular mechanisms of a particular heterozygous mutation in a recessive PD gene. Based on genetic and clinical evaluation as well as functional and structural characterization, we established PINK1 p.G411S as a rare genetic risk factor with a relatively large effect size conferred by a dominant negative function phenotype.
Ämnesord och genrebeteckningar
Biuppslag (personer, institutioner, konferenser, titlar ...)
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Puschmann, A.Lund University,Lunds universitet,Neurologi, Lund,Sektion IV,Institutionen för kliniska vetenskaper, Lund,Medicinska fakulteten,Neurology, Lund,Section IV,Department of Clinical Sciences, Lund,Faculty of Medicine(Swepub:lu)med-aps
(författare)
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Fiesel, Fabienne C
(författare)
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Ando, Maya
(författare)
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Caulfield, Thomas R
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Ogaki, K.
(författare)
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Heckman, M. G.
(författare)
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Hudec, Roman
(författare)
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James, Elle D
(författare)
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Opala, G.
(författare)
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Siuda, J
(författare)
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Boczarska-Jedynak, M.
(författare)
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Friedman, A.
(författare)
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Koziorowski, Dariusz
(författare)
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Aasly, Jan O.
(författare)
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Lynch, T.
(författare)
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Mellick, G. D.
(författare)
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Mohan, M.
(författare)
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Silburn, P. A.
(författare)
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Sanotsky, Y
(författare)
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Vilariño-Güell, C
(författare)
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Farrer, M J
(författare)
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Dawson, T M
(författare)
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Wszolek, Z K
(författare)
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Ross, O A
(författare)
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Neurologi, LundSektion IV
(creator_code:org_t)
Sammanhörande titlar
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Ingår i:Movement Disorders: Wiley31:Suppl. S2, s. 282-2820885-3185
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Till lärosätets databas
- Av författaren/redakt...
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Springer, W
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Puschmann, A.
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Fiesel, Fabienne ...
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Ando, Maya
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Caulfield, Thoma ...
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Ogaki, K.
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visa fler...
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Heckman, M. G.
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Hudec, Roman
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James, Elle D
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Opala, G.
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Siuda, J
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Boczarska-Jedyna ...
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Friedman, A.
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Koziorowski, Dar ...
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Aasly, Jan O.
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Lynch, T.
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Mellick, G. D.
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Mohan, M.
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Silburn, P. A.
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Sanotsky, Y
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Vilariño-Güell, ...
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Farrer, M J
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Dawson, T M
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Wszolek, Z K
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Ross, O A
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visa färre...
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- MEDICIN OCH HÄLSOVETENSKAP
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MEDICIN OCH HÄLS ...
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och Medicinska och f ...
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och Medicinsk geneti ...
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Movement Disorde ...
- Av lärosätet
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Lunds universitet