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Nasal Challenge wit...
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Ekman, Anna-KarinKarolinska Institutet,Lund University,Lunds universitet,Klinisk och experimentell allergiforskning,Laryngoesofagologi, allergi och livskvalitet,Forskargrupper vid Lunds universitet,Clinical and Experimental Allergy Research,Laryngoesophagology, Allergy and Life Quality,Lund University Research Groups
(författare)
Nasal Challenge with LPS Stimulates the Release of Macrophage Inflammatory Protein 1 alpha
- Artikel/kapitelEngelska2009
Förlag, utgivningsår, omfång ...
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2009-01-06
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S. Karger AG,2009
Nummerbeteckningar
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LIBRIS-ID:oai:lup.lub.lu.se:f4137af8-afcb-4bf9-a8fb-bc13b3107536
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https://lup.lub.lu.se/record/1374978URI
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https://doi.org/10.1159/000189199DOI
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http://kipublications.ki.se/Default.aspx?queryparsed=id:118280622URI
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Språk:engelska
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Sammanfattning på:engelska
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Ämneskategori:art swepub-publicationtype
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Ämneskategori:ref swepub-contenttype
Anmärkningar
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Background: Bacterial infections can cause a variety of airway diseases. Toll-like receptors (TLRs) directly respond to the presence of microbes and partake in the innate immune defense. TLR4 is activated by lipopolysaccharide (LPS), and has been detected in sinonasal tissue, epithelial cells and various inflammatory cells. Macrophage inflammatory protein 1 alpha (MIP-1 alpha) is a chemokine released during the inflammatory process. The present study investigated the potential role and regulation of MIP-1 alpha in LPS-induced nasal inflammation. Methods: Thirty-two healthy individuals were intranasally challenged with LPS or vehicle. Nasal lavage was performed, followed by a nasal biopsy. Inflammatory cells were counted, MIP-1 alpha levels analyzed and expression of MIP-1 alpha mRNA in biopsies quantified. Neutrophils isolated from peripheral blood were treated with LPS and effects on MIP1 alpha release, cell survival, and the involved signal pathways, were investigated. Results: LPS challenge caused an increase of MIP-1 alpha in nasal lavage. No corresponding change in mRNA expression was seen in nasal biopsies, suggesting the increase was not due to epithelial synthesis. Neutrophil numbers increased after LPS provocation. Treatment of isolated neutrophils with LPS delayed neutrophil apoptosis and resulted in a time-and concentration-dependent release of MIP-1 alpha, which was reduced by inhibitors of transcription and of nuclear factor (NF)-kappa B, protein kinase C (PKC) and p38 MAPK pathways. Conclusions: Nasal LPS challenge results in release of MIP-1 alpha. The release most likely originates from recruited neutrophils, via NF-kappa B-, PKC-and p38 MAPK-dependent pathways. LPS stimulation delayed neutrophil apop tosis. MIP-1 alpha may constitute an important mediator in neutrophilic airway disease. Copyright (C) 2009 S. Karger AG, Basel
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Biuppslag (personer, institutioner, konferenser, titlar ...)
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Fransson, MattiasLund University,Lunds universitet,Laryngoesofagologi, allergi och livskvalitet,Forskargrupper vid Lunds universitet,Laryngoesophagology, Allergy and Life Quality,Lund University Research Groups(Swepub:lu)oron-mfr
(författare)
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Rydberg, CamillaKarolinska Institutet
(författare)
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Adner, MikaelKarolinska Institutet,Lund University,Lunds universitet,Klinisk och experimentell allergiforskning,Laryngoesofagologi, allergi och livskvalitet,Forskargrupper vid Lunds universitet,Clinical and Experimental Allergy Research,Laryngoesophagology, Allergy and Life Quality,Lund University Research Groups(Swepub:lu)oron-mad
(författare)
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Cardell, Lars-OlafKarolinska Institutet,Lund University,Lunds universitet,Klinisk och experimentell allergiforskning,Laryngoesofagologi, allergi och livskvalitet,Forskargrupper vid Lunds universitet,Clinical and Experimental Allergy Research,Laryngoesophagology, Allergy and Life Quality,Lund University Research Groups(Swepub:lu)oron-lca
(författare)
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Klinisk och experimentell allergiforskningLaryngoesofagologi, allergi och livskvalitet
(creator_code:org_t)
Sammanhörande titlar
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Ingår i:International Archives of Allergy and Immunology: S. Karger AG149:2, s. 154-1601423-00971018-2438
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