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Rat arterial smooth muscle devoid of ryanodine receptor function: effects on cellular Ca2+ handling

Dreja, Karl (author)
Lund University,Lunds universitet,Kärlfysiologi,Forskargrupper vid Lunds universitet,Institutionen för kliniska vetenskaper, Malmö,Medicinska fakulteten,Vascular Physiology,Lund University Research Groups,Department of Clinical Sciences, Malmö,Faculty of Medicine
Nordström, Ina (author)
Lund University,Lunds universitet,Cellulär biomekanik,Forskargrupper vid Lunds universitet,Kärlfysiologi,Cellular Biomechanics,Lund University Research Groups,Vascular Physiology
Hellstrand, Per (author)
Lund University,Lunds universitet,Kärlfysiologi,Forskargrupper vid Lunds universitet,Vascular Physiology,Lund University Research Groups
 (creator_code:org_t)
2009-01-29
2001
English.
In: British Journal of Pharmacology. - : Wiley. - 1476-5381 .- 0007-1188. ; 132:8, s. 1957-1966
  • Journal article (peer-reviewed)
Abstract Subject headings
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  • The roles of intracellular Ca2+ stores and ryanodine (Ry) receptors for vascular Ca2+ homeostasis and viability were investigated in rat tail arterial segments kept in organ culture with Ry (10 100 M) for up to 4 days. Acute exposure to Ry or the non-deactivating ryanodine analogue C10-Oeq glycyl ryanodine (10 M) eliminated Ca2+ release responses to caffeine (20 mM) and noradrenaline (NA, 10 M), whereas responses to NA, but not caffeine, gradually returned to normal within 4 days of exposure to Ry. Ry receptor protein was detected on Western blots in arteries cultured either with or without Ry. Brief Ca2+ release events (sparks) were absent after culture with Ry, whereas Ca2+ waves still occurred. The propagation velocity of waves was equal (19 m s-1) in tissue cultured either with or without Ry. Inhibition of Ca2+ accumulation into the sarcoplasmic reticulum (SR) by culture with caffeine (5 mM), cyclopiazonic acid or thapsigargin (both 10 M) decreased contractility due to Ca2+-induced cell damage. In contrast, culture with Ry did not affect contractility. Removal of Ca2+ from the cytosol following a Ca2+ load was retarded after Ry culture. Thapsigargin reduced the rate of Ca2+ removal in control cultured rings, but had no effect after Ry culture. It is concluded that intracellular Ca2+ stores recover during chronic Ry treatment, while Ry receptors remain non-functional. Ry receptor activity is required for Ca2+ sparks and for SR-dependent recovery from a Ca2+ load, but not for Ca2+ waves or basal Ca2+ homeostasis.

Subject headings

MEDICIN OCH HÄLSOVETENSKAP  -- Medicinska och farmaceutiska grundvetenskaper -- Farmakologi och toxikologi (hsv//swe)
MEDICAL AND HEALTH SCIENCES  -- Basic Medicine -- Pharmacology and Toxicology (hsv//eng)

Keyword

Ryanodine receptors
smooth muscle
Ca2+ stores
sarcoplasmic reticulum
organ culture
Ca2+ sparks
Ca2+ waves

Publication and Content Type

art (subject category)
ref (subject category)

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Dreja, Karl
Nordström, Ina
Hellstrand, Per
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MEDICAL AND HEALTH SCIENCES
MEDICAL AND HEAL ...
and Basic Medicine
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Lund University

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