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Type 1 fimbrial exp...
Type 1 fimbrial expression enhances Escherichia coli virulence for the urinary tract
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- Connell, H. (författare)
- Lund University,Lunds universitet,ITM:s kansli,Institutionen för translationell medicin,Medicinska fakulteten,Dept of Translational Medicine's adminstrative office,Department of Translational Medicine,Faculty of Medicine
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- Agace, W. (författare)
- Lund University,Lunds universitet,Slemhinnans immunologi,Forskargrupper vid Lunds universitet,Mucosal Immunology,Lund University Research Groups
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- Klemm, P. (författare)
- Technical University of Denmark
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- Schembri, M. (författare)
- Technical University of Denmark
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- Mårild, S. (författare)
- University of Gothenburg
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- Svanborg, C. (författare)
- Lund University,Lunds universitet,Avdelningen för mikrobiologi, immunologi och glykobiologi - MIG,Institutionen för laboratoriemedicin,Medicinska fakulteten,Division of Microbiology, Immunology and Glycobiology - MIG,Department of Laboratory Medicine,Faculty of Medicine
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(creator_code:org_t)
- 1996-09-03
- 1996
- Engelska 6 s.
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Ingår i: Proceedings of the National Academy of Sciences of the United States of America. - : Proceedings of the National Academy of Sciences. - 0027-8424. ; 93:18, s. 9827-9832
- Relaterad länk:
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http://dx.doi.org/10...
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http://www.pnas.org/...
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https://lup.lub.lu.s...
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https://doi.org/10.1...
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Abstract
Ämnesord
Stäng
- Type 1 fimbriae are adhesion organelles expressed by many Gram-negative bacteria. They facilitate adherence to mucosal surfaces and inflammatory cells in vitro, but their contribution to virulence has not been defined. This study presents evidence that type 1 fimbriae increase the virulence of Escherichia coli for the urinary tract by promoting bacterial persistence and enhancing the inflammatory response to infection. In a clinical study, we observed that disease severity was greater in children infected with E. coli O1:K1:H7 isolates expressing type 1 fimbriae than in those infected with type 1 negative isolates of the same serotype. The E. coli O1:K1:H7 isolates had the same electrophoretic type, were hemolysin-negative, expressed P fimbriae, and carried the fim DNA sequences. When tested in a mouse urinary tract infection model, the type 1-positive E. coli O1:K1:H7 isolates survived in higher numbers, and induced a greater neutrophil influx into the urine, than O1:K1:H7 type 1-negative isolates. To confirm a role of type 1 fimbriae, a fimH null mutant (CNI016) was constructed from an O1:K1:H7 type 1-positive parent. E. coli CNI016 had reduced survival and inflammatogenicity in the mouse urinary tract infection model. E. coli CNI016 reconstituted with type 1 fimbriae (E. coli CN1018) had restored virulence similar to that of the wild- type parent strain. These results show that type 1 fimbriae in the genetic background of a uropathogenic strain contribute to the pathogenesis of E. coli in the urinary tract.
Ämnesord
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Mikrobiologi inom det medicinska området (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Microbiology in the medical area (hsv//eng)
Nyckelord
- adherence
- inflammation
- neutrophil influx
- urinary tract infection
Publikations- och innehållstyp
- art (ämneskategori)
- ref (ämneskategori)
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