Search: id:"swepub:oai:lup.lub.lu.se:f6e35e4b-7b36-4ab4-acb7-52364fbd46af" >
Cell-Free DNA Promo...
-
Saravanan, RathiNanyang Technological University
(author)
Cell-Free DNA Promotes Thrombin Autolysis and Generation of Thrombin-Derived C-Terminal Fragments
- Article/chapterEnglish2021
Publisher, publication year, extent ...
-
2021-02-24
-
Frontiers Media SA,2021
Numbers
-
LIBRIS-ID:oai:lup.lub.lu.se:f6e35e4b-7b36-4ab4-acb7-52364fbd46af
-
https://lup.lub.lu.se/record/f6e35e4b-7b36-4ab4-acb7-52364fbd46afURI
-
https://doi.org/10.3389/fimmu.2021.593020DOI
Supplementary language notes
-
Language:English
-
Summary in:English
Part of subdatabase
Classification
-
Subject category:art swepub-publicationtype
-
Subject category:ref swepub-contenttype
Notes
-
Cell-free DNA (cfDNA) is the major structural component of neutrophil extracellular traps (NETs), an innate immune response to infection. Antimicrobial proteins and peptides bound to cfDNA play a critical role in the bactericidal property of NETs. Recent studies have shown that NETs have procoagulant activity, wherein cfDNA triggers thrombin generation through activation of the intrinsic pathway of coagulation. We have recently shown that thrombin binds to NETs in vitro and consequently can alter the proteome of NETs. However, the effect of NETs on thrombin is still unknown. In this study, we report that DNA binding leads to thrombin autolysis and generation of multiple thrombin-derived C-terminal peptides (TCPs) in vitro. Employing a 25-residue prototypic TCP, GKY25 (GKYGFYTHVFRLKKWIQKVIDQFGE), we show that TCPs bind NETs, thus conferring mutual protection against nuclease and protease degradation. Together, our results demonstrate the complex interplay between coagulation, NET formation, and thrombin cleavage and identify a previously undisclosed mechanism for formation of TCPs.
Subject headings and genre
Added entries (persons, corporate bodies, meetings, titles ...)
-
Choong, Yeu KhaiNanyang Technological University
(author)
-
Lim, Chun HweeLund University,Lunds universitet,Dermatologi och venereologi, Lund,Sektion III,Institutionen för kliniska vetenskaper, Lund,Medicinska fakulteten,Dermatology and Venereology (Lund),Section III,Department of Clinical Sciences, Lund,Faculty of Medicine,Nanyang Technological University(Swepub:lu)je5455ch
(author)
-
Lim, Li MingNanyang Technological University
(author)
-
Petrlova, JitkaLund University,Lunds universitet,Schmidtchen lab,Forskargrupper vid Lunds universitet,Schmidtchen Lab,Lund University Research Groups(Swepub:lu)med-jkp
(author)
-
Schmidtchen, ArturLund University,Lunds universitet,Schmidtchen lab,Forskargrupper vid Lunds universitet,Schmidtchen Lab,Lund University Research Groups,University of Copenhagen,Bispebjerg Hospital(Swepub:lu)derm-asc
(author)
-
Nanyang Technological UniversityDermatologi och venereologi, Lund
(creator_code:org_t)
Related titles
-
In:Frontiers in Immunology: Frontiers Media SA121664-3224
Internet link
Find in a library
To the university's database