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Biosynthesis of the...
Biosynthesis of the sex pheromone component (E,Z)-7,9-Dodecadienyl acetate in the European Grapevine Moth, Lobesia botrana, involving ∆11 desaturation and an elusive ∆7 desaturase
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- Ding, Bao Jian (author)
- Lund University,Lunds universitet,Funktionell zoologi,Biologiska institutionen,Naturvetenskapliga fakulteten,Feromongruppen,Forskargrupper vid Lunds universitet,Functional zoology,Department of Biology,Faculty of Science,Pheromone Group,Lund University Research Groups,Lund Univ, Dept Biol, Solvegatan 37, SE-22362 Lund, Sweden.
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- Xia, Yi Han (author)
- Lund University,Lunds universitet,Funktionell zoologi,Biologiska institutionen,Naturvetenskapliga fakulteten,Feromongruppen,Forskargrupper vid Lunds universitet,Functional zoology,Department of Biology,Faculty of Science,Pheromone Group,Lund University Research Groups,Lund Univ, Dept Biol, Solvegatan 37, SE-22362 Lund, Sweden.
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- Wang, Hong Lei (author)
- Lund University,Lunds universitet,Funktionell zoologi,Biologiska institutionen,Naturvetenskapliga fakulteten,Feromongruppen,Forskargrupper vid Lunds universitet,Functional zoology,Department of Biology,Faculty of Science,Pheromone Group,Lund University Research Groups,Lund Univ, Dept Biol, Solvegatan 37, SE-22362 Lund, Sweden.
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- Andersson, Fredrik (author)
- Mittuniversitetet,Mid Sweden University,Institutionen för kemiteknik
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- Hedenström, Erik, 1955- (author)
- Mittuniversitetet,Mid Sweden University,Institutionen för kemiteknik
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- Gross, Jürgen (author)
- Julius Kühn-Institute Federal Research Centre for Cultivated Plants,Julius Kuhn Inst, Inst Plant Protect Fruit Crops & Viticulture, Fed Res Ctr Cultivated Plants, Dossenheim, Germany.,Julius Kühn-Institut
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- Löfstedt, Christer (author)
- Lund University,Lunds universitet,Funktionell zoologi,Biologiska institutionen,Naturvetenskapliga fakulteten,Feromongruppen,Forskargrupper vid Lunds universitet,Functional zoology,Department of Biology,Faculty of Science,Pheromone Group,Lund University Research Groups,Lund Univ, Dept Biol, Solvegatan 37, SE-22362 Lund, Sweden.
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- Ding, Baojian (author)
- Lunds universitet,Lund University
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- Xia, Yihan, 1990 (author)
- Lunds universitet,Lund University
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- Wang, Honglei (author)
- Lunds universitet,Lund University
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(creator_code:org_t)
- 2021-03-29
- 2021
- English.
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In: Journal of Chemical Ecology. - : Springer Science and Business Media LLC. - 0098-0331 .- 1573-1561. ; 47:3, s. 248-264
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Abstract
Subject headings
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- The European grapevine moth, Lobesia botrana, uses (E,Z)-7,9-dodecadienyl acetate as its major sex pheromone component. Through in vivo labeling experiments we demonstrated that the doubly unsaturated pheromone component is produced by ∆11 desaturation of tetradecanoic acid, followed by chain shortening of (Z)-11-tetradecenoic acid to (Z)-9-dodecenoic acid, and subsequently introduction of the second double bond by an unknown ∆7 desaturase, before final reduction and acetylation. By sequencing and analyzing the transcriptome of female pheromone glands of L. botrana, we obtained 41 candidate genes that may be involved in sex pheromone production, including the genes encoding 17 fatty acyl desaturases, 13 fatty acyl reductases, 1 fatty acid synthase, 3 acyl-CoA oxidases, 1 acetyl-CoA carboxylase, 4 fatty acid transport proteins and 2 acyl-CoA binding proteins. A functional assay of desaturase and acyl-CoA oxidase gene candidates in yeast and insect cell (Sf9) heterologous expression systems revealed that Lbo_PPTQ encodes a ∆11 desaturase producing (Z)-11-tetradecenoic acid from tetradecanoic acid. Further, Lbo_31670 and Lbo_49602 encode two acyl-CoA oxidases that may produce (Z)-9-dodecenoic acid by chain shortening (Z)-11-tetradecenoic acid. The gene encoding the enzyme introducing the E7 double bond into (Z)-9-dodecenoic acid remains elusive even though we assayed 17 candidate desaturases in the two heterologous systems.
Subject headings
- NATURVETENSKAP -- Biologi -- Biokemi och molekylärbiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Biochemistry and Molecular Biology (hsv//eng)
- NATURVETENSKAP -- Biologi -- Zoologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Zoology (hsv//eng)
- NATURVETENSKAP -- Kemi -- Analytisk kemi (hsv//swe)
- NATURAL SCIENCES -- Chemical Sciences -- Analytical Chemistry (hsv//eng)
- NATURVETENSKAP -- Biologi -- Ekologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Ecology (hsv//eng)
Keyword
- Acyl-CoA oxidase
- Chain shortening
- Gene functional characterization
- In vivo labeling experiment
- Pheromone gland
- Transcriptome
- In vivo labeling experiments
- transcriptome
- gene functional analysis
- Acyl-CoA oxidase
- chain shortening
- Acyl-CoA oxidase
Publication and Content Type
- art (subject category)
- ref (subject category)
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- By the author/editor
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Ding, Bao Jian
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Xia, Yi Han
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Wang, Hong Lei
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Andersson, Fredr ...
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Hedenström, Erik ...
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Gross, Jürgen
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Löfstedt, Christ ...
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Ding, Baojian
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Xia, Yihan, 1990
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Wang, Honglei
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- About the subject
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- NATURAL SCIENCES
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NATURAL SCIENCES
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and Biological Scien ...
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and Biochemistry and ...
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- NATURAL SCIENCES
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NATURAL SCIENCES
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and Biological Scien ...
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and Zoology
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- NATURAL SCIENCES
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NATURAL SCIENCES
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and Chemical Science ...
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and Analytical Chemi ...
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- NATURAL SCIENCES
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NATURAL SCIENCES
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and Biological Scien ...
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and Ecology
- Articles in the publication
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Journal of Chemi ...
- By the university
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Lund University
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Mid Sweden University
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Chalmers University of Technology