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  • Lien, Tonje G.Oslo university hospital (author)

Sample preparation approach influences pam50 risk of recurrence score in early breast cancer

  • Article/chapterEnglish2021

Publisher, publication year, extent ...

  • 2021-12-04
  • MDPI AG,2021

Numbers

  • LIBRIS-ID:oai:lup.lub.lu.se:fac8f3a4-29d4-461b-b3e6-241877dbda13
  • https://lup.lub.lu.se/record/fac8f3a4-29d4-461b-b3e6-241877dbda13URI
  • https://doi.org/10.3390/cancers13236118DOI

Supplementary language notes

  • Language:English
  • Summary in:English

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Classification

  • Subject category:art swepub-publicationtype
  • Subject category:ref swepub-contenttype

Notes

  • The PAM50 gene expression subtypes and the associated risk of recurrence (ROR) score are used to predict the risk of recurrence and the benefits of adjuvant therapy in early-stage breast cancer. The Prosigna assay includes the PAM50 subtypes along with their clinicopathological fea-tures, and is approved for treatment recommendations for adjuvant hormonal therapy and chemotherapy in hormone-receptor-positive early breast cancer. The Prosigna test utilizes RNA extracted from macrodissected tumor cells obtained from formalin-fixed, paraffin-embedded (FFPE) tissue sections. However, RNA extracted from fresh-frozen (FF) bulk tissue without macrodissection is widely used for research purposes, and yields high-quality RNA for downstream analyses. To in-vestigate the impact of the sample preparation approach on ROR scores, we analyzed 94 breast carcinomas included in an observational study that had available gene expression data from macro-dissected FFPE tissue and FF bulk tumor tissue, along with the clinically approved Prosigna scores for the node-negative, hormone-receptor-positive, HER2-negative cases (n = 54). ROR scores were calculated in R; the resulting two sets of scores from FFPE and FF samples were compared, and treatment recommendations were evaluated. Overall, ROR scores calculated based on the macro-dissected FFPE tissue were consistent with the Prosigna scores. However, analyses from bulk tissue yielded a higher proportion of cases classified as normal-like; these were samples with relatively low tumor cellularity, leading to lower ROR scores. When comparing ROR scores (low, intermedi-ate, and high), discordant cases between the two preparation approaches were revealed among the luminal tumors; the recommended treatment would have changed in a minority of cases.

Subject headings and genre

Added entries (persons, corporate bodies, meetings, titles ...)

  • Ohnstad, Hege OmaOslo university hospital (author)
  • Lingjærde, Ole ChristianOslo university hospital,University of Oslo (author)
  • Vallon-Christersson, JohanLund University,Lunds universitet,Bröstcancer-genetik,Sektion I,Institutionen för kliniska vetenskaper, Lund,Medicinska fakulteten,LUCC: Lunds universitets cancercentrum,Övriga starka forskningsmiljöer,Breastcancer-genetics,Section I,Department of Clinical Sciences, Lund,Faculty of Medicine,LUCC: Lund University Cancer Centre,Other Strong Research Environments(Swepub:lu)onk-jvc (author)
  • Aaserud, MaritOslo university hospital (author)
  • Sveli, My Anh TuOslo university hospital (author)
  • Borg, ÅkeLund University,Lunds universitet,Bröstcancer-genetik,Sektion I,Institutionen för kliniska vetenskaper, Lund,Medicinska fakulteten,Familjär bröstcancer,Forskargrupper vid Lunds universitet,LUCC: Lunds universitets cancercentrum,Övriga starka forskningsmiljöer,Breastcancer-genetics,Section I,Department of Clinical Sciences, Lund,Faculty of Medicine,Familial Breast Cancer,Lund University Research Groups,LUCC: Lund University Cancer Centre,Other Strong Research Environments(Swepub:lu)onk-abo (author)
  • Garred, ØysteinOslo university hospital (author)
  • Borgen, ElinOslo university hospital (author)
  • Naume, BjørnOslo university hospital,University of Oslo (author)
  • Russnes, HegeOslo university hospital (author)
  • Sørlie, ThereseUniversity of Oslo,Oslo university hospital (author)
  • Oslo university hospitalUniversity of Oslo (creator_code:org_t)
  • on behalf of OSBREAC

Related titles

  • In:Cancers: MDPI AG13:232072-6694

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