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Protocol for optica...
Protocol for optical clearing and imaging of fluorescently labeled ex vivo rat brain slices
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- Giacomoni, Jessica (author)
- Lund University,Lunds universitet,Utvecklings- och regenerativ neurobiologi,Forskargrupper vid Lunds universitet,Developmental and Regenerative Neurobiology,Lund University Research Groups
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- Habekost, Mette (author)
- Lund University,Lunds universitet,Utvecklings- och regenerativ neurobiologi,Forskargrupper vid Lunds universitet,Developmental and Regenerative Neurobiology,Lund University Research Groups,University of Copenhagen
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- Cepeda-Prado, Efrain (author)
- Lund University,Lunds universitet,Regenerativ neurofysiologi,Forskargrupper vid Lunds universitet,Regenerative Neurophysiology,Lund University Research Groups
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- Mattsson, Bengt (author)
- Lund University,Lunds universitet,Utvecklings- och regenerativ neurobiologi,Forskargrupper vid Lunds universitet,Developmental and Regenerative Neurobiology,Lund University Research Groups
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- Rylander Ottosson, Daniella (author)
- Lund University,Lunds universitet,Regenerativ neurofysiologi,Forskargrupper vid Lunds universitet,Regenerative Neurophysiology,Lund University Research Groups
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- Parmar, Malin (author)
- Lund University,Lunds universitet,Utvecklings- och regenerativ neurobiologi,Forskargrupper vid Lunds universitet,Developmental and Regenerative Neurobiology,Lund University Research Groups
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- Kajtez, Janko (author)
- Lund University,Lunds universitet,Utvecklings- och regenerativ neurobiologi,Forskargrupper vid Lunds universitet,Developmental and Regenerative Neurobiology,Lund University Research Groups
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(creator_code:org_t)
- Elsevier BV, 2023
- 2023
- English.
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In: STAR Protocols. - : Elsevier BV. - 2666-1667. ; 4:1
- Related links:
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http://dx.doi.org/10... (free)
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https://lup.lub.lu.s...
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https://doi.org/10.1...
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Abstract
Subject headings
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- Tissue clearing is commonly used for whole-brain imaging but seldom used for brain slices. Here, we present a simple protocol to slice, immunostain, and clear sections of adult rat brains for subsequent high-resolution confocal imaging. The protocol does not require toxic reagents or specialized equipment. We also provide instructions for culturing of rat brain slices free floating on permeable culture inserts, maintained in regular CO2 incubators, and handled only at media change.
Subject headings
- TEKNIK OCH TEKNOLOGIER -- Medicinteknik -- Annan medicinteknik (hsv//swe)
- ENGINEERING AND TECHNOLOGY -- Medical Engineering -- Other Medical Engineering (hsv//eng)
Publication and Content Type
- art (subject category)
- ref (subject category)
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