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Tomosyn-1 is involv...
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Cheviet, Severine
(författare)
Tomosyn-1 is involved in a post-docking event required for pancreatic beta-cell exocytosis
- Artikel/kapitelEngelska2006
Förlag, utgivningsår, omfång ...
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The Company of Biologists,2006
Nummerbeteckningar
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LIBRIS-ID:oai:lup.lub.lu.se:fc7890dc-0f2c-49c7-b98c-3f65ebc7957f
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https://lup.lub.lu.se/record/404115URI
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https://doi.org/10.1242/jcs.03037DOI
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Språk:engelska
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Sammanfattning på:engelska
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Ämneskategori:art swepub-publicationtype
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Ämneskategori:ref swepub-contenttype
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Although the assembly of a ternary complex between the SNARE proteins syntaxin-1, SNAP25 and VAMP2 is known to be crucial for insulin exocytosis, the mechanisms controlling this key event are poorly understood. We found that pancreatic beta-cells express different isoforms of tomosyn-1, a syntaxin-1-binding protein possessing a SNARE-like motif. Using atomic force microscopy we show that the SNARE-like domain of tomosyn-1 can form a complex with syntaxin-1 and SNAP25 but displays binding forces that are weaker than those observed for VAMP2 (237 +/- 13 versus 279 +/- 3 pN). In pancreatic beta-cells tomosyn-1 was found to be concentrated in cellular compartments enriched in insulin-containing secretory granules. Silencing of tomosyn-1 in the rat beta-cell line INS-1E by RNA interference did not affect the number of secretory granules docked at the plasma membrane but led to a reduction in stimulus-induced exocytosis. Replacement of endogenous tomosyn-1 with mouse tomosyn-1, which differs in the nucleotide sequence from its rat homologue and escapes silencing, restored a normal secretory rate. Taken together, our data suggest that tomosyn-1 is involved in a post-docking event that prepares secretory granules for fusion and is necessary to sustain exocytosis of pancreatic beta-cells in response to insulin secretagogues.
Ämnesord och genrebeteckningar
Biuppslag (personer, institutioner, konferenser, titlar ...)
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Bezzi, Paola
(författare)
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Ivarsson, RositaLund University,Lunds universitet,Diabetes - öpatofysiologi,Forskargrupper vid Lunds universitet,Diabetes - Islet Patophysiology,Lund University Research Groups(Swepub:lu)mphy-riv
(författare)
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Renström, ErikLund University,Lunds universitet,Diabetes - öpatofysiologi,Forskargrupper vid Lunds universitet,Diabetes - Islet Patophysiology,Lund University Research Groups(Swepub:lu)mphy-ere
(författare)
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Viertl, David
(författare)
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Kasas, Sandor
(författare)
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Catsicas, Stefan
(författare)
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Regazzi, Romano
(författare)
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Diabetes - öpatofysiologiForskargrupper vid Lunds universitet
(creator_code:org_t)
Sammanhörande titlar
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Ingår i:Journal of Cell Science: The Company of Biologists119:14, s. 2912-29200021-95331477-9137
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