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- Lohmander, StefanLund University,Lunds universitet,Ortopedi, Lund,Sektion III,Institutionen för kliniska vetenskaper, Lund,Medicinska fakulteten,Lund OsteoArthritis Division - Nedbrytning av ledbrosk: en biologisk process som leder till artros,Forskargrupper vid Lunds universitet,Orthopaedics (Lund),Section III,Department of Clinical Sciences, Lund,Faculty of Medicine,Lund OsteoArthritis Division - Molecular marker research group,Lund University Research Groups (author)
Analysis by high-performance liquid chromatography of radioactively labeled carbohydrate components of proteoglycans
- Article/chapterEnglish1986
Publisher, publication year, extent ...
- Elsevier BV,1986
- 10 s.
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- LIBRIS-ID:oai:lup.lub.lu.se:fc90eeeb-55b3-4487-93ce-ac0e74c492bc
- https://lup.lub.lu.se/record/fc90eeeb-55b3-4487-93ce-ac0e74c492bcURI
- https://doi.org/10.1016/0003-2697(86)90498-7DOI
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- Language:English
- Summary in:English
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- Methods were developed for the separation of radioactively labeled carbohydrate components of proteoglycans by isocratic ion-moderated partition HPLC. Neutral sugars were separated after hydrolysis in trifluoroacetic acid with baseline separation between glucose, xylose, galactose, fucose, and mannose. N-Acetylneuraminic acid, N-acetylated hexosamines, glucose, galactose, and xylitol were likewise well separated from each other under isocratic elution conditions. Glucuronic acid, iduronic acid, and their lactones were separated after hydrolysis in formic acid and sulfuric acid. Glucosamine, galactosamine, galactosaminitol, and glucosaminitol were separated by HPLC on a cation exchanger with neutral buffer after hydrolysis in hydrochloric acid. The separation techniques also proved useful in fractionation of exoglycosidase digests of O- and N-linked oligosaccharides. Separations of aldoses, hexosamines, and uronic acids were adapted to sensitive photometric detection.
Subject headings and genre
- MEDICIN OCH HÄLSOVETENSKAP Medicinska och farmaceutiska grundvetenskaper Cell- och molekylärbiologi hsv//swe
- MEDICAL AND HEALTH SCIENCES Basic Medicine Cell and Molecular Biology hsv//eng
- glycoproteins
- HPLC carbohydrates
- liquid scintillation counting
- polysaccharides
- protein processing
- sugar analysis
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- Ortopedi, LundSektion III (creator_code:org_t)
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- In:Analytical Biochemistry: Elsevier BV154:1, s. 75-840003-2697
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