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Proteomic analysis ...
Proteomic analysis of brain metastatic lung adenocarcinoma reveals intertumoral heterogeneity and specific alterations associated with the timing of brain metastases
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- Woldmar, N. (author)
- Lund University,Lunds universitet,LUCC: Lunds universitets cancercentrum,Övriga starka forskningsmiljöer,LUCC: Lund University Cancer Centre,Other Strong Research Environments,Federal University of Rio de Janeiro
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- Schwendenwein, A. (author)
- Medical University of Vienna
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- Kuras, M. (author)
- Lund University,Lunds universitet,Klinisk kemi, Malmö,Forskargrupper vid Lunds universitet,Clinical Protein Science and Imaging,Clinical Chemistry, Malmö,Lund University Research Groups
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- Szeitz, B. (author)
- Semmelweis University
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- Boettiger, K. (author)
- Medical University of Vienna
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- Tisza, A. (author)
- Semmelweis University,National Korányi Institute for Tuberculosis and Pulmonology, Hungary
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- László, V. (author)
- National Korányi Institute for Tuberculosis and Pulmonology, Hungary,Medical University of Vienna
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- Reiniger, L. (author)
- Semmelweis University,Hungarian Academy of Sciences
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Bagó, A. G. (author)
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- Szállási, Z. (author)
- Danish Cancer Society,Harvard Medical School,Hungarian Academy of Sciences
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- Moldvay, J. (author)
- Hungarian Academy of Sciences,National Korányi Institute for Tuberculosis and Pulmonology, Hungary
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- Szász, A. M. (author)
- National Korányi Institute for Tuberculosis and Pulmonology, Hungary,Semmelweis University
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- Malm, J. (author)
- Lund University,Lunds universitet,Klinisk kemi, Malmö,Forskargrupper vid Lunds universitet,Clinical Chemistry, Malmö,Lund University Research Groups,Skåne University Hospital
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- Horvatovich, P. (author)
- University of Groningen
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- Pizzatti, L. (author)
- Federal University of Rio de Janeiro
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- Domont, G. B. (author)
- Federal University of Rio de Janeiro
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- Rényi-Vámos, F. (author)
- Semmelweis University,National Korányi Institute for Tuberculosis and Pulmonology, Hungary,National Institute of Oncology, Budapest
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- Hoetzenecker, K. (author)
- Medical University of Vienna
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- Hoda, M. A. (author)
- Medical University of Vienna
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- Marko-Varga, G. (author)
- Lund University,Lunds universitet,Avdelningen för Biomedicinsk teknik,Institutionen för biomedicinsk teknik,Institutioner vid LTH,Lunds Tekniska Högskola,Department of Biomedical Engineering,Departments at LTH,Faculty of Engineering, LTH
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- Schelch, K. (author)
- Medical University of Vienna
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- Megyesfalvi, Z. (author)
- National Korányi Institute for Tuberculosis and Pulmonology, Hungary,Semmelweis University,Medical University of Vienna,National Institute of Oncology, Budapest
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- Rezeli, M. (author)
- Lund University,Lunds universitet,Avdelningen för Biomedicinsk teknik,Institutionen för biomedicinsk teknik,Institutioner vid LTH,Lunds Tekniska Högskola,Department of Biomedical Engineering,Departments at LTH,Faculty of Engineering, LTH
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- Döme, B. (author)
- Semmelweis University,National Institute of Oncology, Budapest,Medical University of Vienna,Skåne University Hospital,Lund University,National Korányi Institute for Tuberculosis and Pulmonology, Hungary
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(creator_code:org_t)
- Elsevier BV, 2023
- 2023
- English.
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In: ESMO Open. - : Elsevier BV. - 2059-7029. ; 8:1
- Related links:
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http://dx.doi.org/10... (free)
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https://lup.lub.lu.s...
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https://doi.org/10.1...
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Abstract
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- Background: Brain metastases are associated with considerable negative effects on patients’ outcome in lung adenocarcinoma (LADC). Here, we investigated the proteomic landscape of primary LADCs and their corresponding brain metastases. Materials and methods: Proteomic profiling was conducted on 20 surgically resected primary and brain metastatic LADC samples via label-free shotgun proteomics. After sample processing, peptides were analyzed using an Ultimate 3000 pump coupled to a QExactive HF-X mass spectrometer. Raw data were searched using PD 2.4. Further data analyses were carried out using Perseus, RStudio and GraphPad Prism. Proteomic data were correlated with clinical and histopathological parameters and the timing of brain metastases. Mass spectrometry-based proteomic data are available via ProteomeXchange with identifier PXD027259. Results: Out of the 6821 proteins identified and quantified, 1496 proteins were differentially expressed between primary LADCs and corresponding brain metastases. Pathways associated with the immune system, cell-cell/matrix interactions and migration were predominantly activated in the primary tumors, whereas pathways related to metabolism, translation or vesicle formation were overrepresented in the metastatic tumors. When comparing fast- versus slow-progressing patients, we found 454 and 298 differentially expressed proteins in the primary tumors and brain metastases, respectively. Metabolic reprogramming and ribosomal activity were prominently up-regulated in the fast-progressing patients (versus slow-progressing individuals), whereas expression of cell-cell interaction- and immune system-related pathways was reduced in these patients and in those with multiple brain metastases. Conclusions: This is the first comprehensive proteomic analysis of paired primary tumors and brain metastases of LADC patients. Our data suggest a malfunction of cellular attachment and an increase in ribosomal activity in LADC tissue, promoting brain metastasis. The current study provides insights into the biology of LADC brain metastases and, moreover, might contribute to the development of personalized follow-up strategies in LADC.
Subject headings
- MEDICIN OCH HÄLSOVETENSKAP -- Klinisk medicin -- Cancer och onkologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Clinical Medicine -- Cancer and Oncology (hsv//eng)
Keyword
- brain metastasis
- clinical proteomics
- intertumoral heterogeneity
- lung adenocarcinoma
Publication and Content Type
- art (subject category)
- ref (subject category)
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ESMO Open
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To the university's database
- By the author/editor
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Woldmar, N.
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Schwendenwein, A ...
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Kuras, M.
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Szeitz, B.
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Boettiger, K.
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Tisza, A.
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show more...
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László, V.
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Reiniger, L.
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Bagó, A. G.
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Szállási, Z.
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Moldvay, J.
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Szász, A. M.
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Malm, J.
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Horvatovich, P.
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Pizzatti, L.
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Domont, G. B.
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Rényi-Vámos, F.
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Hoetzenecker, K.
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Hoda, M. A.
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Marko-Varga, G.
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Schelch, K.
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Megyesfalvi, Z.
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Rezeli, M.
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Döme, B.
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- About the subject
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- MEDICAL AND HEALTH SCIENCES
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MEDICAL AND HEAL ...
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and Clinical Medicin ...
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and Cancer and Oncol ...
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ESMO Open
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Lund University