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Chromosome 19 Annot...
Chromosome 19 Annotations with Disease Speciation: A First Report from the Global Research Consortium
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Nilsson, C. L. (author)
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Berven, F. (author)
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Selheim, F. (author)
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show more...
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- Lindberg, Henrik (author)
- Lund University,Lunds universitet,Avdelningen för Biomedicinsk teknik,Institutionen för biomedicinsk teknik,Institutioner vid LTH,Lunds Tekniska Högskola,Department of Biomedical Engineering,Departments at LTH,Faculty of Engineering, LTH
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Moskal, J. R. (author)
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Kroes, R. A. (author)
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Sulman, E. P. (author)
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Conrad, C. A. (author)
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Lang, F. F. (author)
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- Andren, Per E. (author)
- Uppsala universitet,Institutionen för farmaceutisk biovetenskap
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- Nilsson, Anna (author)
- Uppsala universitet,Institutionen för farmaceutisk biovetenskap
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- Carlsohn, Elisabet (author)
- Gothenburg University,Göteborgs universitet,Core Facilities, Proteomics,Core Facilities, Proteomics
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Lilja, H. (author)
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- Malm, Johan (author)
- Lund University,Lunds universitet,Klinisk kemi, Malmö,Forskargrupper vid Lunds universitet,Clinical Chemistry, Malmö,Lund University Research Groups
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Fenyo, D. (author)
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Subramaniyam, D. (author)
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Wang, X. D. (author)
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Gonzales-Gonzales, M. (author)
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Dasilva, N. (author)
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Diez, P. (author)
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Fuentes, M. (author)
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- Végvári, Ákos (author)
- Lund University,Lunds universitet,Avdelningen för Biomedicinsk teknik,Institutionen för biomedicinsk teknik,Institutioner vid LTH,Lunds Tekniska Högskola,Department of Biomedical Engineering,Departments at LTH,Faculty of Engineering, LTH
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- Sjödin, Karin (author)
- Lund University,Lunds universitet,Avdelningen för Biomedicinsk teknik,Institutionen för biomedicinsk teknik,Institutioner vid LTH,Lunds Tekniska Högskola,Department of Biomedical Engineering,Departments at LTH,Faculty of Engineering, LTH
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- Welinder, Charlotte (author)
- Lund University,Lunds universitet,Tumörmikromiljö,Sektion I,Institutionen för kliniska vetenskaper, Lund,Medicinska fakulteten,Tumor microenvironment,Section I,Department of Clinical Sciences, Lund,Faculty of Medicine
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- Laurell, Thomas (author)
- Lund University,Lunds universitet,Avdelningen för Biomedicinsk teknik,Institutionen för biomedicinsk teknik,Institutioner vid LTH,Lunds Tekniska Högskola,Department of Biomedical Engineering,Departments at LTH,Faculty of Engineering, LTH
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- Fehniger, Thomas (author)
- Lund University,Lunds universitet,Avdelningen för Biomedicinsk teknik,Institutionen för biomedicinsk teknik,Institutioner vid LTH,Lunds Tekniska Högskola,Department of Biomedical Engineering,Departments at LTH,Faculty of Engineering, LTH
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Lindberg, H. (author)
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- Rezeli, Melinda (author)
- Lund University,Lunds universitet,Avdelningen för Biomedicinsk teknik,Institutionen för biomedicinsk teknik,Institutioner vid LTH,Lunds Tekniska Högskola,Department of Biomedical Engineering,Departments at LTH,Faculty of Engineering, LTH
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Edula, G. (author)
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- Hober, Sophia (author)
- KTH,Proteomik
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- Marko-Varga, György (author)
- Lund University,Lunds universitet,Avdelningen för Biomedicinsk teknik,Institutionen för biomedicinsk teknik,Institutioner vid LTH,Lunds Tekniska Högskola,Department of Biomedical Engineering,Departments at LTH,Faculty of Engineering, LTH
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Liu, Huiling (author)
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(creator_code:org_t)
- 2012-12-18
- 2013
- English.
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In: Journal of Proteome Research. - : American Chemical Society (ACS). - 1535-3893 .- 1535-3907. ; 12:1, s. 134-149
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Abstract
Subject headings
Close
- A first research development progress report of the Chromosome 19 Consortium with members from Sweden, Norway, Spain, United States, China and India, a part of the Chromosome-centric Human Proteome Project (C-HPP) global initiative, is presented (http://www.c-hpp.org). From the chromosome 19 peptide-targeted library constituting 6159 peptides, a pilot study was conducted using a subset with 125 isotope-labeled peptides. We applied an annotation strategy with triple quadrupole, ESI-Qtrap, and MALDI mass spectrometry platforms, comparing the quality of data within and in between these instrumental set-ups. LC–MS conditions were outlined by multiplex assay developments, followed by MRM assay developments. SRM was applied to biobank samples, quantifying kallikrein 3 (prostate specific antigen) in plasma from prostate cancer patients. The antibody production has been initiated for more than 1200 genes from the entire chromosome 19, and the progress developments are presented. We developed a dedicated transcript microarray to serve as the mRNA identifier by screening cancer cell lines. NAPPA protein arrays were built to align with the transcript data with the Chromosome 19 NAPPA chip, dedicated to 90 proteins, as the first development delivery. We have introduced an IT-infrastructure utilizing a LIMS system that serves as the key interface for the research teams to share and explore data generated within the project. The cross-site data repository will form the basis for sample processing, including biological samples as well as patient samples from national Biobanks.
Subject headings
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Medicinsk genetik (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Medical Genetics (hsv//eng)
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Farmaceutiska vetenskaper (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Pharmaceutical Sciences (hsv//eng)
- NATURVETENSKAP -- Biologi -- Genetik (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Genetics (hsv//eng)
- NATURVETENSKAP -- Biologi -- Biokemi och molekylärbiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Biochemistry and Molecular Biology (hsv//eng)
- TEKNIK OCH TEKNOLOGIER -- Medicinteknik (hsv//swe)
- ENGINEERING AND TECHNOLOGY -- Medical Engineering (hsv//eng)
Keyword
- proteins
- genes
- antibodies
- mRNA
- mass spectrometry
- bioinformatics
- protein microarray
- human
- human proteome project
- gene-expression
- hybridization
- guidelines
- arrays
- Proteins
- Genes
- Antibodies
- Mass spectrometry
- Bioinformatics
- Protein microarray
- Human Disease
- proteins
Publication and Content Type
- ref (subject category)
- art (subject category)
Find in a library
To the university's database
- By the author/editor
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Nilsson, C. L.
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Berven, F.
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Selheim, F.
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Lindberg, Henrik
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Moskal, J. R.
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Kroes, R. A.
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show more...
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Sulman, E. P.
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Conrad, C. A.
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Lang, F. F.
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Andren, Per E.
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Nilsson, Anna
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Carlsohn, Elisab ...
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Lilja, H.
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Malm, Johan
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Fenyo, D.
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Subramaniyam, D.
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Wang, X. D.
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Gonzales-Gonzale ...
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Dasilva, N.
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Diez, P.
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Fuentes, M.
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Végvári, Ákos
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Sjödin, Karin
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Welinder, Charlo ...
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Laurell, Thomas
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Fehniger, Thomas
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Lindberg, H.
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Rezeli, Melinda
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Edula, G.
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Hober, Sophia
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Marko-Varga, Gyö ...
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Liu, Huiling
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show less...
- About the subject
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- MEDICAL AND HEALTH SCIENCES
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MEDICAL AND HEAL ...
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and Basic Medicine
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and Medical Genetics
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- MEDICAL AND HEALTH SCIENCES
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MEDICAL AND HEAL ...
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and Basic Medicine
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and Pharmaceutical S ...
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- NATURAL SCIENCES
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NATURAL SCIENCES
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and Biological Scien ...
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and Genetics
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- NATURAL SCIENCES
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NATURAL SCIENCES
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and Biological Scien ...
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and Biochemistry and ...
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- ENGINEERING AND TECHNOLOGY
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ENGINEERING AND ...
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and Medical Engineer ...
- Articles in the publication
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Journal of Prote ...
- By the university
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University of Gothenburg
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Lund University
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Royal Institute of Technology
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Uppsala University