X-chromosome upregulation is driven by increased burst frequency

2019-10-03
Stockholm :Karolinska Institutet, Dept of Medical Biochemistry and Biophysics,2019
electronicrdacarrier

Ohno's hypothesis postulates that X-chromosome upregulation rectifies X-dose imbalance relative to autosomal genes, present in two active copies per cell. Here we dissected X-upregulation into kinetics of transcription, inferred from allele-specific single-cell RNA-sequencing (scRNAseq) data from somatic mouse cells. We confirmed increased X-chromosome expression in female and male somatic cells, and remarkably found that the X-chromosome achieved upregulation by elevated burst frequencies. By monitoring differentiating female embryonic stem cells, we found that elevated burst frequency established on the active X-chromosome as X-inactivation occurred on the other allele. This provides mechanistic insights into X-chromosome upregulation.

Ingår i:Nature Structural & Molecular BiologyStockholm : Karolinska Institutet, Dept of Medical Biochemistry and Biophysics1545-99931545-9985

Av författaren/redakt...: Larsson, Anton J ...; Coucoravas, Chri ...; Sandberg, Rickar ...; Reinius, Björn

Kungliga biblioteket hanterar dina personuppgifter i enlighet med EU:s dataskyddsförordning (2018), GDPR. Läs mer om hur det funkar här.
Så här hanterar KB dina uppgifter vid användning av denna tjänst.