id:"swepub:oai:prod.swepub.kib.ki.se:118997876"
Sökning: id:"swepub:oai:prod.swepub.kib.ki.se:118997876" > Molecular species o...
- 1 av 1
- Föregående post
- Nästa post
- Till träfflistan
- Helander, AKarolinska Institutet (författare)
Molecular species of the alcohol biomarker phosphatidylethanol in human blood measured by LC-MS
- Artikel/kapitelEngelska2009
Förlag, utgivningsår, omfång ...
- 2009-07-01
- Oxford University Press (OUP),2009
Nummerbeteckningar
- LIBRIS-ID:oai:prod.swepub.kib.ki.se:118997876
- http://kipublications.ki.se/Default.aspx?queryparsed=id:118997876URI
- https://doi.org/10.1373/clinchem.2008.120923DOI
Kompletterande språkuppgifter
- Språk:engelska
- Sammanfattning på:engelska
Ingår i deldatabas
- SwePubSwePub
Klassifikation
Anmärkningar
- Background: The alcohol biomarker phosphatidylethanol (PEth) comprises a group of ethanol-derived phospholipids formed from phosphatidylcholine by phospholipase D. The PEth molecular species have a common phosphoethanol head group onto which 2 fatty acid moieties are attached. We developed an electrospray ionization (ESI) LC-MS method for qualitative and quantitative measurement of different PEth species in human blood.Methods: We subjected a total lipid extract of whole blood to HPLC gradient separation on a C4 column and performed LC-ESI-MS analysis using selected ion monitoring of deprotonated molecules for the PEth species and phosphatidylpropanol (internal standard). Identification of individual PEth species was based on ESI–tandem mass spectrometry (MS/MS) analysis of product ions.Results: The fatty acid moieties were the major product ions of PEth, based on comparison with PEth-16:0/16:0, 18:1/18:1, and 16:0/18:1 reference material. For LC-MS analysis of different PEth species in blood, we used a calibration curve covering 0.2–7.0 μmol/L PEth-16:0/18:1. The lower limit of quantitation of the method was <0.1 μmol/L, and intra- and interassay CVs were <9% and <11%. In blood samples collected from 38 alcohol patients, the total PEth concentration ranged between 0.1 and 21.7 μmol/L (mean 8.9). PEth-16:0/18:1 and 16:0/18:2 were the predominant molecular species, accounting for approximately 37% and 25%, respectively, of total PEth. PEth-16:0/20:4 and mixtures of 18:1/18:1 plus 18:0/18:2 (not separated using selected ion monitoring because of identical molecular masses) and 16:0/20:3 plus 18:1/18.2 made up approximately 13%, 12%, and 8%.Conclusions: This LC-MS method allows simultaneous qualitative and quantitative measurement of several PEth molecular species in whole blood samples.
Biuppslag (personer, institutioner, konferenser, titlar ...)
- Zheng, YF (författare)
- Karolinska Institutet (creator_code:org_t)
Sammanhörande titlar
- Ingår i:Clinical chemistry: Oxford University Press (OUP)55:7, s. 1395-14051530-85610009-9147
Internetlänk
- 1 av 1
- Föregående post
- Nästa post
- Till träfflistan
Hitta mer i SwePub
- Av författaren/redakt...
- Helander, A
- Zheng, YF
- Artiklar i publikationen
- Clinical chemist ...
- Av lärosätet
- Karolinska Institutet
Sök utanför SwePub
- Sök vidare i:
- Google Book Search
- Google Scholar
Kungliga biblioteket hanterar dina personuppgifter i enlighet med EU:s dataskyddsförordning (2018), GDPR. Läs mer om hur det funkar här.
Så här hanterar KB dina uppgifter vid användning av denna tjänst.
- Copyright © LIBRIS - Nationella bibliotekssystem
- LIBRIS.kb.se
