Sökning: id:"swepub:oai:prod.swepub.kib.ki.se:12128586" >
Effects of palmitat...
-
Fauconnier, J
(författare)
Effects of palmitate on Ca(2+) handling in adult control and ob/ob cardiomyocytes: impact of mitochondrial reactive oxygen species
- Artikel/kapitelEngelska2007
Förlag, utgivningsår, omfång ...
-
American Diabetes Association,2007
Nummerbeteckningar
-
LIBRIS-ID:oai:prod.swepub.kib.ki.se:12128586
-
http://kipublications.ki.se/Default.aspx?queryparsed=id:12128586URI
-
https://doi.org/10.2337/db06-0739DOI
Kompletterande språkuppgifter
-
Språk:engelska
-
Sammanfattning på:engelska
Ingår i deldatabas
Klassifikation
-
Ämneskategori:ref swepub-contenttype
-
Ämneskategori:art swepub-publicationtype
Anmärkningar
-
Obesity and insulin resistance are associated with enhanced fatty acid utilization, which may play a central role in diabetic cardiomyopathy. We now assess the effect of the saturated fatty acid palmitate (1.2 mmol/l) on Ca2+ handling, cell shortening, and mitochondrial production of reactive oxygen species (ROS) in freshly isolated ventricular cardiomyocytes from normal (wild-type) and obese, insulin-resistant ob/ob mice. Cardiomyocytes were electrically stimulated at 1 Hz, and the signal of fluorescent indicators was measured with confocal microscopy. Palmitate decreased the amplitude of cytosolic Ca2+ transients (measured with fluo-3), the sarcoplasmic reticulum Ca2+ load, and cell shortening by ∼20% in wild-type cardiomyocytes; these decreases were prevented by the general antioxidant N-acetylcysteine. In contrast, palmitate accelerated Ca2+ transients and increased cell shortening in ob/ob cardiomyocytes. Application of palmitate rapidly dissipated the mitochondrial membrane potential (measured with tetra-methyl rhodamine-ethyl ester) and increased the mitochondrial ROS production (measured with MitoSOX Red) in wild-type but not in ob/ob cardiomyocytes. In conclusion, increased saturated fatty acid levels impair cellular Ca2+ handling and contraction in a ROS-dependent manner in normal cardiomyocytes. Conversely, high fatty acid levels may be vital to sustain cardiac Ca2+ handling and contraction in obesity and insulin-resistant conditions.
Biuppslag (personer, institutioner, konferenser, titlar ...)
-
Andersson, DCKarolinska Institutet
(författare)
-
Zhang, SJKarolinska Institutet
(författare)
-
Lanner, JTKarolinska Institutet
(författare)
-
Wibom, RKarolinska Institutet
(författare)
-
Katz, AKarolinska Institutet
(författare)
-
Bruton, JDKarolinska Institutet
(författare)
-
Westerblad, HKarolinska Institutet
(författare)
-
Karolinska Institutet
(creator_code:org_t)
Sammanhörande titlar
-
Ingår i:Diabetes: American Diabetes Association56:4, s. 1136-11420012-17971939-327X
Internetlänk
Hitta via bibliotek
-
Diabetes
(Sök värdpublikationen i LIBRIS)
Till lärosätets databas