Clonal culturing of human embryonic stem cells on laminin-521/E-cadherin matrix in defined and xeno-free environment

Lack of robust methods for establishment and expansion of pluripotent human embryonic stem (hES) cells still hampers development of cell therapy. Laminins (LN) are a family of highly cell-type specific basement membrane proteins important for cell adhesion, differentiation, migration and phenotype stability. Here we produce and isolate a human recombinant LN-521 isoform and develop a cell culture matrix containing LN-521 and E-cadherin, which both localize to stem cell niches in vivo. This matrix allows clonal derivation, clonal survival and long-term self-renewal of hES cells under completely chemically defined and xeno-free conditions without ROCK inhibitors. Neither LN-521 nor E-cadherin alone enable clonal survival of hES cells. The LN-521/E-cadherin matrix allows hES cell line derivation from blastocyst inner cell mass and single blastomere cells without a need to destroy the embryo. This method can facilitate the generation of hES cell lines for development of different cell types for regenerative medicine purposes.

Ingår i:Nature communications: Springer Science and Business Media LLC5, s. 3195-2041-1723

Av författaren/redakt...: Rodin, S; Antonsson, L; Niaudet, C; Simonson, OE; Salmela, E; Hansson, EM; visa fler...; Domogatskaya, A; Xiao, ZJ; Damdimopoulou, P; Sheikhi, M; Inzunza, J; Nilsson, AS; Baker, D; Kuiper, R; Sun, Y; Blennow, E; Nordenskjold, M; Grinnemo, KH; Kere, J; Betsholtz, C; Hovatta, O; Tryggvason, K; visa färre...

Kungliga biblioteket hanterar dina personuppgifter i enlighet med EU:s dataskyddsförordning (2018), GDPR. Läs mer om hur det funkar här.
Så här hanterar KB dina uppgifter vid användning av denna tjänst.