Additive effects of microRNAs and transcription factors on CCL2 production in human white adipose tissue

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Kulyte, AKarolinska Institutet (author)

Additive effects of microRNAs and transcription factors on CCL2 production in human white adipose tissue

Article/chapterEnglish2014

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2014-03-13
American Diabetes Association,2014

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LIBRIS-ID:oai:prod.swepub.kib.ki.se:129211402
http://kipublications.ki.se/Default.aspx?queryparsed=id:129211402URI
https://doi.org/10.2337/db13-0702DOI

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Language:English
Summary in:English

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Subject category:ref swepub-contenttype
Subject category:art swepub-publicationtype

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Adipose tissue inflammation is present in insulin-resistant conditions. We recently proposed a network of microRNAs (miRNAs) and transcription factors (TFs) regulating the production of the proinflammatory chemokine (C-C motif) ligand-2 (CCL2) in adipose tissue. We presently extended and further validated this network and investigated if the circuits controlling CCL2 can interact in human adipocytes and macrophages. The updated subnetwork predicted that miR-126/-193b/-92a control CCL2 production by several TFs, including v-ets erythroblastosis virus E26 oncogene homolog 1 (avian) (ETS1), MYC-associated factor X (MAX), and specificity protein 12 (SP1). This was confirmed in human adipocytes by the observation that gene silencing of ETS1, MAX, or SP1 attenuated CCL2 production. Combined gene silencing of ETS1 and MAX resulted in an additive reduction in CCL2 production. Moreover, overexpression of miR-126/-193b/-92a in different pairwise combinations reduced CCL2 secretion more efficiently than either miRNA alone. However, although effects on CCL2 secretion by co-overexpression of miR-92a/-193b and miR-92a/-126 were additive in adipocytes, the combination of miR-126/-193b was primarily additive in macrophages. Signals for miR-92a and -193b converged on the nuclear factor-κB pathway. In conclusion, TF and miRNA-mediated regulation of CCL2 production is additive and partly relayed by cell-specific networks in human adipose tissue that may be important for the development of insulin resistance/type 2 diabetes.

Added entries (persons, corporate bodies, meetings, titles ...)

Belarbi, Y (author)
Lorente-Cebrian, S (author)
Bambace, C (author)
Arner, E (author)
Daub, COKarolinska Institutet (author)
Heden, P (author)
Ryden, MKarolinska Institutet (author)
Mejhert, NKarolinska Institutet (author)
Arner, PKarolinska Institutet (author)
Karolinska Institutet (creator_code:org_t)

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In:Diabetes: American Diabetes Association63:4, s. 1248-12581939-327X0012-1797

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By the author/editor: Kulyte, A; Belarbi, Y; Lorente-Cebrian, ...; Bambace, C; Arner, E; Daub, CO; show more...; Heden, P; Ryden, M; Mejhert, N; Arner, P; show less...

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