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Identification of N...
Identification of Novel Epigenetic Markers of Prostate Cancer by NotI-Microarray Analysis
- Article/chapterEnglish2015
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LIBRIS-ID:oai:prod.swepub.kib.ki.se:132186248
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http://kipublications.ki.se/Default.aspx?queryparsed=id:132186248URI
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https://doi.org/10.1155/2015/241301DOI
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Language:English
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Summary in:English
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Subject category:ref swepub-contenttype
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Subject category:art swepub-publicationtype
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A significant need for reliable and accurate cancer diagnostics and prognosis compels the search for novel biomarkers that would be able to discriminate between indolent and aggressive tumors at the early stages of disease. The aim of this work was identification of potential diagnostic biomarkers for characterization of different types of prostate tumors. NotI-microarrays with 180 clones associated with chromosome 3 genes/loci were applied to determine genetic and epigenetic alterations in 33 prostate tumors. For 88 clones, aberrations were detected in more than 10% of tumors. The major types of alterations were DNA methylation and/or deletions. Frequent methylation of the discovered loci was confirmed by bisulfite sequencing on selective sampling of genes:FGF12,GATA2, andLMCD1. Three genes (BHLHE40,BCL6, andITGA9) were tested for expression level alterations using qPCR, and downregulation associated with hypermethylation was shown in the majority of tumors. Based on these data, we proposed the set of potential biomarkers for detection of prostate cancer and discrimination between prostate tumors with different malignancy and aggressiveness:BHLHE40,FOXP1,LOC285205,ITGA9,CTDSPL,FGF12,LOC440944/SETD5,VHL,CLCN2,OSBPL10/ZNF860,LMCD1,FAM19A4,CAND2,MAP4,KY, andLRRC58. Moreover, we probabilistically estimated putative functional relations between the genes within each set using the network enrichment analysis.
Added entries (persons, corporate bodies, meetings, titles ...)
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Rosenberg, EE
(author)
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Krasnov, GS
(author)
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Gerashchenko, GV
(author)
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Gordiyuk, VV
(author)
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Pavlova, TVKarolinska Institutet
(author)
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Kudryavtseva, AV
(author)
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Beniaminov, AD
(author)
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Belova, AA
(author)
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Bondarenko, YN
(author)
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Danilets, RO
(author)
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Glukhov, AI
(author)
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Kondratov, AG
(author)
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Alexeyenko, AKarolinska Institutet
(author)
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Alekseev, BY
(author)
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Klein, GKarolinska Institutet
(author)
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Senchenko, VN
(author)
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Kashuba, VIKarolinska Institutet
(author)
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Karolinska Institutet
(creator_code:org_t)
Related titles
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In:Disease markers: Hindawi Limited2015, s. 241301-1875-86300278-0240
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- By the author/editor
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Dmitriev, AA
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Rosenberg, EE
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Krasnov, GS
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Gerashchenko, GV
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Gordiyuk, VV
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Pavlova, TV
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show more...
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Kudryavtseva, AV
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Beniaminov, AD
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Belova, AA
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Bondarenko, YN
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Danilets, RO
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Glukhov, AI
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Kondratov, AG
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Alexeyenko, A
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Alekseev, BY
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Klein, G
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Senchenko, VN
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Kashuba, VI
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- Articles in the publication
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Disease markers
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Karolinska Institutet