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Clustering of confo...
Clustering of conformational IgE epitopes on the major dog allergen Can f 1
- Article/chapterEnglish2017
Publisher, publication year, extent ...
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2017-09-22
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Springer Science and Business Media LLC,2017
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LIBRIS-ID:oai:prod.swepub.kib.ki.se:136684074
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http://kipublications.ki.se/Default.aspx?queryparsed=id:136684074URI
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https://doi.org/10.1038/s41598-017-11672-5DOI
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Language:English
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Summary in:English
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Subject category:ref swepub-contenttype
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Subject category:art swepub-publicationtype
Notes
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Immunoglobulin E (IgE)-associated allergy affects more than 25% of the population. Can f 1 is the major dog allergen associated with respiratory symptoms but the epitopes recognized by allergic patients IgE on Can f 1 are unknown. To characterize IgE epitopes of Can f 1 recognized by dog allergic patients, six overlapping peptides spanning the Can f 1 sequence were synthesized. In direct IgE epitope mapping experiments peptides were analyzed for IgE reactivity by dot blot and Enzyme-linked immunosorbent assay (ELISA) with sera from dog allergic patients. For indirect epitope-mapping, rabbits were immunized with the peptides to generate specific IgG antibodies which were used to inhibit allergic patients’ IgE binding to Can f 1. IgE binding sites were visualized on a model of the Can f 1 three-dimensional structure. We found that Can f 1 does not contain any relevant sequential IgE epitopes. However, IgE inhibition experiments with anti-peptide specific IgGs showed that Can f 1 N- and C-terminal portion assembled a major conformational binding site. In conclusion, our study is the first to identify the major IgE epitope-containing area of the dog allergen Can f 1. This finding is important for the development of allergen-specific treatment strategies.
Added entries (persons, corporate bodies, meetings, titles ...)
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Weber, M
(author)
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Hofer, G
(author)
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Apostolovic, DKarolinska Institutet
(author)
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Keller, W
(author)
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Reininger, R
(author)
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Swoboda, I
(author)
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Spitzauer, S
(author)
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Focke-Tejkl, M
(author)
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van Hage, MKarolinska Institutet
(author)
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Valenta, R
(author)
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Karolinska Institutet
(creator_code:org_t)
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In:Scientific reports: Springer Science and Business Media LLC7:1, s. 12135-2045-2322
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Curin, M
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Weber, M
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Hofer, G
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Apostolovic, D
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Keller, W
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Reininger, R
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show more...
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Swoboda, I
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Spitzauer, S
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Focke-Tejkl, M
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van Hage, M
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Valenta, R
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Scientific repor ...
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Karolinska Institutet