Sökning: WFRF:(Wu YW)
> (2015-2019) >
The Effect of Serto...
The Effect of Sertoli Cells on Xenotransplantation and Allotransplantation of Ventral Mesencephalic Tissue in a Rat Model of Parkinson's Disease
-
Jhao, YT (författare)
-
Chiu, CH (författare)
-
Chen, CFF (författare)
-
visa fler...
-
Chou, TK (författare)
-
Lin, YW (författare)
-
Ju, YT (författare)
-
Wu, SC (författare)
-
Yan, RF (författare)
-
Shiue, CY (författare)
-
Chueh, SH (författare)
-
- Halldin, C (författare)
- Karolinska Institutet
-
Cheng, CY (författare)
-
Ma, KH (författare)
-
visa färre...
-
(creator_code:org_t)
- 2019-11-11
- 2019
- Engelska.
-
Ingår i: Cells. - : MDPI AG. - 2073-4409. ; 8:11
- Relaterad länk:
-
https://www.mdpi.com...
-
visa fler...
-
http://kipublication...
-
https://doi.org/10.3...
-
visa färre...
Abstract
Ämnesord
Stäng
- Intra-striatal transplantation of fetal ventral mesencephalic (VM) tissue has a therapeutic effect on patients with Parkinson’s disease (PD). Sertoli cells (SCs) possess immune-modulatory properties that benefit transplantation. We hypothesized that co-graft of SCs with VM tissue can attenuate rejection. Hemi-parkinsonian rats were generated by injecting 6-hydroxydopamine into the right medial forebrain bundle of Sprague Dawley (SD) rats. The rats were then intrastriatally transplanted with VM tissue from rats or pigs (rVM or pVM), with/without a co-graft of SCs (rVM+SCs or pVM+SCs). Recovery of dopaminergic function and survival of the grafts were evaluated using the apomorphine-induced rotation test and small animal-positron emission tomography (PET) coupled with [18F] DOPA or [18F] FE-PE2I, respectively. Immunohistochemistry (IHC) examination was used to determine the survival of the grafted dopaminergic neurons in the striatum and to investigate immune-modulatory effects of SCs. The results showed that the rVM+SCs and pVM+SCs groups had significantly improved drug-induced rotational behavior compared with the VM alone groups. PET revealed a significant increase in specific uptake ratios (SURs) of [18F] DOPA and [18F] FE-PE2I in the grafted striatum of the rVM+SCs and pVM+SCs groups as compared to that of the rVM and pVM groups. SC and VM tissue co-graft led to better dopaminergic (DA) cell survival. The co-grafted groups exhibited lower populations of T-cells and activated microglia compared to the groups without SCs. Our results suggest that co-graft of SCs benefit both xeno- and allo-transplantation of VM tissue in a PD rat model. Use of SCs enhanced the survival of the grafted dopaminergic neurons and improved functional recovery. The enhancement may in part be attributable to the immune-modulatory properties of SCs. In addition, [18F]DOPA and [18F]FE-PE2I coupled with PET may provide a feasible method for in vivo evaluation of the functional integrity of the grafted DA cell in parkinsonian rats.
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
Hitta via bibliotek
-
Cells
(Sök värdpublikationen i LIBRIS)
Till lärosätets databas
- Av författaren/redakt...
-
Jhao, YT
-
Chiu, CH
-
Chen, CFF
-
Chou, TK
-
Lin, YW
-
Ju, YT
-
visa fler...
-
Wu, SC
-
Yan, RF
-
Shiue, CY
-
Chueh, SH
-
Halldin, C
-
Cheng, CY
-
Ma, KH
-
visa färre...
- Artiklar i publikationen
-
Cells
- Av lärosätet
-
Karolinska Institutet