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Human Pluripotent S...
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Kurek, MKarolinska Institutet
(author)
Human Pluripotent Stem Cells in Reproductive Science-a Comparison of Protocols Used to Generate and Define Male Germ Cells from Pluripotent Stem Cells
- Article/chapterEnglish2020
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LIBRIS-ID:oai:prod.swepub.kib.ki.se:143500933
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http://kipublications.ki.se/Default.aspx?queryparsed=id:143500933URI
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https://doi.org/10.3390/ijms21031028DOI
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Language:English
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Summary in:English
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Subject category:ref swepub-contenttype
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Subject category:art swepub-publicationtype
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Globally, fertility-related issues affect around 15% of couples. In 20%–30% of cases men are solely responsible, and they contribute in around 50% of all cases. Hence, understanding of in vivo germ-cell specification and exploring different angles of fertility preservation and infertility intervention are considered hot topics nowadays, with special focus on the use of human pluripotent stem cells (hPSCs) as a source of in vitro germ-cell generation. However, the generation of male germ cells from hPSCs can currently be considered challenging, making a judgment on the real perspective of these innovative approaches difficult. Ever since the first spontaneous germ-cell differentiation studies, using human embryonic stem cells, various strategies, including specific co-cultures, gene over-expression, and addition of growth factors, have been applied for human germ-cell derivation. In line with the variety of differentiation methods, the outcomes have ranged from early and migratory primordial germ cells up to post-meiotic spermatids. This variety of culture approaches and cell lines makes comparisons between protocols difficult. Considering the diverse strategies and outcomes, we aim in this mini-review to summarize the literature regarding in vitro derivation of human male germ cells from hPSCs, while keeping a particular focus on the culture methods, growth factors, and cell lines used.
Added entries (persons, corporate bodies, meetings, titles ...)
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Albalushi, H
(author)
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Hovatta, O
(author)
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Stukenborg, JBKarolinska Institutet
(author)
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Karolinska Institutet
(creator_code:org_t)
Related titles
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In:International journal of molecular sciences: MDPI AG21:31422-0067
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