SwePub
Sök i LIBRIS databas

  Utökad sökning

onr:"swepub:oai:prod.swepub.kib.ki.se:143853858"
 

Sökning: onr:"swepub:oai:prod.swepub.kib.ki.se:143853858" > Follicular hormone ...

  • Poulsen, LC (författare)

Follicular hormone dynamics during the midcycle surge of gonadotropins in women undergoing fertility treatment

  • Artikel/kapitelEngelska2020

Förlag, utgivningsår, omfång ...

  • 2020-02-05
  • Oxford University Press (OUP),2020

Nummerbeteckningar

  • LIBRIS-ID:oai:prod.swepub.kib.ki.se:143853858
  • http://kipublications.ki.se/Default.aspx?queryparsed=id:143853858URI
  • https://doi.org/10.1093/molehr/gaaa013DOI

Kompletterande språkuppgifter

  • Språk:engelska
  • Sammanfattning på:engelska

Ingår i deldatabas

Klassifikation

  • Ämneskategori:ref swepub-contenttype
  • Ämneskategori:art swepub-publicationtype

Anmärkningar

  • Changes in concentrations of intra-follicular hormones during ovulation are important for final oocyte maturation and endometrial priming to ensure reproductive success. As no human studies have investigated these changes in detail, our objective was to describe the dynamics of major follicular fluid (FF) hormones and transcription of steroidogenic enzymes and steroid receptors in human granulosa cells (GCs) during ovulation. We conducted a prospective cohort study at a public fertility clinic in 2016–2018. Fifty women undergoing ovarian stimulation for fertility treatment were included. From each woman, FF and GCs were collected by transvaginal ultrasound-guided follicle puncture of one follicle at two specific time points during ovulation, and the study covered a total of five time points: before ovulation induction (OI), 12, 17, 32 and 36 h after OI. Follicular fluid concentrations of oestradiol, progesterone, androstenedione, testosterone, 17-hydroxyprogesterone, anti-Mullerian hormone, inhibin A and inhibin B were measured using ELISA assays, and a statistical mixed model was used to analyse differences in hormone levels between time points. Gene expression of 33 steroidogenic enzymes and six hormone receptors in GCs across ovulation were assessed by microarray analysis, and selected genes were validated by quantitative reverse transcription PCR. We found that concentrations of oestradiol, testosterone, progesterone, AMH, inhibin A and inhibin B (P < 0.001) and gene expression of 12 steroidogenic enzymes and five receptors (false discovery rate < 0.0001) changed significantly during ovulation. Furthermore, we found parallel changes in plasma hormones. The substantial changes in follicular hormone production during ovulation highlight their importance for reproductive success.

Biuppslag (personer, institutioner, konferenser, titlar ...)

  • Englund, ALM (författare)
  • Andersen, AS (författare)
  • Botkjaer, JA (författare)
  • Mamsen, LS (författare)
  • Damdimopoulou, PKarolinska Institutet (författare)
  • Ostrup, O (författare)
  • Grondahl, ML (författare)
  • Yding Andersen, C (författare)
  • Karolinska Institutet (creator_code:org_t)

Sammanhörande titlar

  • Ingår i:Molecular human reproduction: Oxford University Press (OUP)26:4, s. 256-2681460-2407

Internetlänk

Hitta via bibliotek

Till lärosätets databas

Kungliga biblioteket hanterar dina personuppgifter i enlighet med EU:s dataskyddsförordning (2018), GDPR. Läs mer om hur det funkar här.
Så här hanterar KB dina uppgifter vid användning av denna tjänst.

 
pil uppåt Stäng

Kopiera och spara länken för att återkomma till aktuell vy