Search: id:"swepub:oai:prod.swepub.kib.ki.se:144744856" >
Massive and rapid C...
-
Smyrlaki, IKarolinska Institutet
(author)
Massive and rapid COVID-19 testing is feasible by extraction-free SARS-CoV-2 RT-PCR
- Article/chapterEnglish2020
Publisher, publication year, extent ...
-
2020-09-23
-
Springer Science and Business Media LLC,2020
Numbers
-
LIBRIS-ID:oai:prod.swepub.kib.ki.se:144744856
-
http://kipublications.ki.se/Default.aspx?queryparsed=id:144744856URI
-
https://doi.org/10.1038/s41467-020-18611-5DOI
Supplementary language notes
-
Language:English
-
Summary in:English
Part of subdatabase
Classification
-
Subject category:ref swepub-contenttype
-
Subject category:art swepub-publicationtype
Notes
-
Coronavirus disease 2019 (COVID-19), caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), is commonly diagnosed by reverse transcription polymerase chain reaction (RT-PCR) to detect viral RNA in patient samples, but RNA extraction constitutes a major bottleneck in current testing. Methodological simplification could increase diagnostic availability and efficiency, benefitting patient care and infection control. Here, we describe methods circumventing RNA extraction in COVID-19 testing by performing RT-PCR directly on heat-inactivated or lysed samples. Our data, including benchmarking using 597 clinical patient samples and a standardised diagnostic system, demonstrate that direct RT-PCR is viable option to extraction-based tests. Using controlled amounts of active SARS-CoV-2, we confirm effectiveness of heat inactivation by plaque assay and evaluate various generic buffers as transport medium for direct RT-PCR. Significant savings in time and cost are achieved through RNA-extraction-free protocols that are directly compatible with established PCR-based testing pipelines. This could aid expansion of COVID-19 testing.
Added entries (persons, corporate bodies, meetings, titles ...)
-
Ekman, M
(author)
-
Lentini, AKarolinska Institutet
(author)
-
de Sousa, NRKarolinska Institutet
(author)
-
Papanicolaou, NKarolinska Institutet
(author)
-
Vondracek, M
(author)
-
Aarum, J
(author)
-
Safari, H
(author)
-
Muradrasoli, S
(author)
-
Rothfuchs, AGKarolinska Institutet
(author)
-
Albert, JKarolinska Institutet
(author)
-
Hogberg, BKarolinska Institutet
(author)
-
Reinius, BKarolinska Institutet
(author)
-
Karolinska Institutet
(creator_code:org_t)
Related titles
-
In:Nature communications: Springer Science and Business Media LLC11:1, s. 4812-2041-1723
Internet link
Find in a library
To the university's database
- By the author/editor
-
Smyrlaki, I
-
Ekman, M
-
Lentini, A
-
de Sousa, NR
-
Papanicolaou, N
-
Vondracek, M
-
show more...
-
Aarum, J
-
Safari, H
-
Muradrasoli, S
-
Rothfuchs, AG
-
Albert, J
-
Hogberg, B
-
Reinius, B
-
show less...
- Articles in the publication
-
Nature communica ...
- By the university
-
Karolinska Institutet