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Human Cytomegalovir...
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Yaiw, KCKarolinska Institutet
(författare)
Human Cytomegalovirus Reduces Endothelin-1 Expression in Both Endothelial and Vascular Smooth Muscle Cells
- Artikel/kapitelEngelska2021
Förlag, utgivningsår, omfång ...
Nummerbeteckningar
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LIBRIS-ID:oai:prod.swepub.kib.ki.se:146977080
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http://kipublications.ki.se/Default.aspx?queryparsed=id:146977080URI
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https://doi.org/10.3390/microorganisms9061137DOI
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Språk:engelska
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Sammanfattning på:engelska
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Ämneskategori:ref swepub-contenttype
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Ämneskategori:art swepub-publicationtype
Anmärkningar
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Human cytomegalovirus (HCMV) is an opportunistic pathogen that has been implicated in the pathogenesis of atherosclerosis. Endothelin-1 (ET-1), a potent vasoconstrictive peptide, is overexpressed and strongly associated with many vasculopathies. The main objective of this study was to investigate whether HCMV could affect ET-1 production. As such, both endothelial and smooth muscle cells, two primary cell types involved in the pathogenesis of atherosclerosis, were infected with HCMV in vitro and ET-1 mRNA and proteins were assessed by quantitative PCR assay, immunofluorescence staining and ELISA. HCMV infection significantly decreased ET-1 mRNA and secreted bioactive ET-1 levels from both cell types and promoted accumulation of the ET-1 precursor protein in infected endothelial cells. This was associated with inhibition of expression of the endothelin converting enzyme-1 (ECE-1), which cleaves the ET-1 precursor protein to mature ET-1. Ganciclovir treatment did not prevent the virus suppressive effects on ET-1 expression. Consistent with this observation we identified that the IE2-p86 protein predominantly modulated ET-1 expression. Whether the pronounced effects of HCMV in reducing ET-1 expression in vitro may lead to consequences for regulation of the vascular tone in vivo remains to be proven.
Biuppslag (personer, institutioner, konferenser, titlar ...)
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Mohammad, AA
(författare)
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Taher, C
(författare)
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Cui, HL
(författare)
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Costa, H
(författare)
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Kostopoulou, ONKarolinska Institutet
(författare)
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Jung, M
(författare)
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Assinger, A
(författare)
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Wilhelmi, V
(författare)
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Yang, JNKarolinska Institutet
(författare)
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Straat, K
(författare)
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Rahbar, AKarolinska Institutet
(författare)
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Pernow, JKarolinska Institutet
(författare)
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Soderberg-Naucler, CKarolinska Institutet
(författare)
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Karolinska Institutet
(creator_code:org_t)
Sammanhörande titlar
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Ingår i:Microorganisms: MDPI AG9:62076-2607
Internetlänk
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Till lärosätets databas
- Av författaren/redakt...
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Yaiw, KC
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Mohammad, AA
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Taher, C
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Cui, HL
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Costa, H
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Kostopoulou, ON
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visa fler...
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Jung, M
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Assinger, A
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Wilhelmi, V
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Yang, JN
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Straat, K
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Rahbar, A
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Pernow, J
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Soderberg-Naucle ...
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visa färre...
- Artiklar i publikationen
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Microorganisms
- Av lärosätet
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Karolinska Institutet