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RT-qPCR assay for d...
RT-qPCR assay for detection of mink astrovirus in outbreaks of diarrhea on Danish mink farms
- Artikel/kapitelEngelska2021
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2021-05-26
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Public Library of Science (PLoS),2021
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LIBRIS-ID:oai:prod.swepub.kib.ki.se:147104537
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http://kipublications.ki.se/Default.aspx?queryparsed=id:147104537URI
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https://doi.org/10.1371/journal.pone.0252022DOI
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Språk:engelska
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Sammanfattning på:engelska
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Ämneskategori:art swepub-publicationtype
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Diarrhea in mink kits is a major cause of disease and mortality in the mink production. The etiology remains unknown in most outbreaks due to a lack of diagnostic assays. In the current study we present an RT-qPCR method to detect mink astrovirus in fecal samples from mink kits with diarrhea. All sampled animals were classified based on age and patoanatomical evaluation as having pre-weaning diarrhea, diarrhea in the growth period or as having no macroscopic signs of diarrhea. Fecal samples were analyzed for MiAstV with RT-qPCR, next generation sequencing and electron microscopy in parallel. Mink astrovirus was detected with RT-qPCR in 92 out of 203 samples. This detection was confirmed by next generation sequencing in a high proportion of samples (22/27), and by visualization of astrovirus particles with EM in some of the samples. Mink astrovirus was highly prevalent (68%) among kits in the outbreaks of pre-weaning diarrhea, in particular outbreaks from May, while less prevalent in outbreaks in June. Mink astrovirus was detected in outbreaks of diarrhea in the growth period, though in a much lesser extent than in the pre-weaning period. The role of mink astrovirus in the diarrhea disease complex of mink remain to be investigated, and for that purpose this sensitive and robust RT-qPCR can be a valuable tool in the future.
Biuppslag (personer, institutioner, konferenser, titlar ...)
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Ullman, K
(författare)
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Leijon, M
(författare)
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Hedlund, KO
(författare)
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Klingstrom, JKarolinska Institutet
(författare)
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Krarup, LI
(författare)
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Andresen, L
(författare)
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Quaade, ML
(författare)
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Hammer, AS
(författare)
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Karolinska Institutet
(creator_code:org_t)
Sammanhörande titlar
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Ingår i:PloS one: Public Library of Science (PLoS)16:5, s. e0252022-1932-6203
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