Sökning: L773:1939 4586 >
ago1 and dcr1, two ...
-
Carmichael, JB
(författare)
ago1 and dcr1, two core components of the RNA interference pathway, functionally diverge from rdp1 in regulating cell cycle events in Schizosaccharomyces pombe
- Artikel/kapitelEngelska2004
Förlag, utgivningsår, omfång ...
-
American Society for Cell Biology (ASCB),2004
Nummerbeteckningar
-
LIBRIS-ID:oai:prod.swepub.kib.ki.se:1934180
-
http://kipublications.ki.se/Default.aspx?queryparsed=id:1934180URI
-
https://doi.org/10.1091/mbc.e03-06-0433DOI
Kompletterande språkuppgifter
-
Språk:engelska
-
Sammanfattning på:engelska
Ingår i deldatabas
Klassifikation
-
Ämneskategori:ref swepub-contenttype
-
Ämneskategori:art swepub-publicationtype
Anmärkningar
-
In the fission yeast Schizosaccharomyces pombe, three genes that function in the RNA interference (RNAi) pathway, ago1+, dcr1+, and rdp1+, have recently been shown to be important for timely formation of heterochromatin and accurate chromosome segregation. In the present study, we present evidence that null mutants for ago1+and dcr1+but not rdp1+, exhibit abnormal cytokinesis, cell cycle arrest deficiencies, and mating defects. Subsequent analyses showed that ago1+and dcr1+are required for regulated hyperphosphorylation of Cdc2 when encountering genotoxic insults. Because rdp1+is dispensable for this process, the functions of ago1+and dcr1+in this pathway are presumably independent of their roles in RNAi-mediated heterochromatin formation and chromosome segregation. This was further supported by the finding that ago1+is a multicopy suppressor of the S-M checkpoint deficiency and cytokinesis defects associated with loss of Dcr1 function, but not for the chromosome segregation defects of this mutant. Accordingly, we conclude that Dcr1-dependent production of small interfering RNAs is not required for enactment and/or maintenance of certain cell cycle checkpoints and that Ago1 and Dcr1 functionally diverge from Rdp1 to control cell cycle events in fission yeast. Finally, exogenous expression of hGERp95/EIF2C2/hAgo2, a human Ago1 homolog implicated in posttranscriptional gene silencing, compensated for the loss of ago1+function in S. pombe. This suggests that PPD proteins may also be important for regulation of cell cycle events in higher eukaryotes.
Biuppslag (personer, institutioner, konferenser, titlar ...)
-
Provost, P
(författare)
-
Ekwall, KKarolinska Institutet
(författare)
-
Hobman, TC
(författare)
-
Karolinska Institutet
(creator_code:org_t)
Sammanhörande titlar
-
Ingår i:Molecular biology of the cell: American Society for Cell Biology (ASCB)15:3, s. 1425-14351059-15241939-4586
Internetlänk
Hitta via bibliotek
Till lärosätets databas