WFRF:(Klein K.)
Sökning: WFRF:(Klein K.) > (1995-1999) > Human herpesvirus-8...
- Szekely, LKarolinska Institutet (författare)
Human herpesvirus-8-encoded LNA-1 accumulates in heterochromatin- associated nuclear bodies
- Artikel/kapitelEngelska1999
Förlag, utgivningsår, omfång ...
- Microbiology Society,1999
Nummerbeteckningar
- LIBRIS-ID:oai:prod.swepub.kib.ki.se:1938498
- http://kipublications.ki.se/Default.aspx?queryparsed=id:1938498URI
- https://doi.org/10.1099/0022-1317-80-11-2889DOI
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- Språk:engelska
- Sammanfattning på:engelska
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Anmärkningar
- Subnuclear distribution of the human herpesvirus-8 (HHV-8)- encoded nuclear protein LNA-1 was analysed at high resolution in body cavity (BC) lymphoma-derived cell lines, in cell hybrids between BC cells and various human and mouse cells and in freshly infected K562 and ECV cell lines. Three-dimensional reconstruction of nuclei from optical sections and quantitative analysis of the distribution of LNA-1 fluorescence in relation to chromatin showed that LNA-1 associates preferentially with the border of heterochromatin in the interphase nuclei. This was further confirmed in the following systems: in endo- and exonuclease-digested nuclei, in human–mouse (BC-1–Sp2- 0) hybrids and on chromatin spreads. LNA-1 was found to bind to mitotic chromosomes at random. Epstein–Barr virus (EBV), but not HHV-8, was rapidly lost from mouse–human hybrid cells in parallel with the loss of human chromosomes. HHV-8 could persist on the residual mouse background for more than 8 months. In early human–mouse hybrids that contain a single fused nucleus, LNA-1 preferentially associates with human chromatin. After the gradual loss of the human chromosomes, LNA-1 becomes associated with the murine pericentromeric heterochromatin. In human–human hybrids derived from the fusion of the HHV-8-carrying BCBL-1 cells and the EBV-immortalized lymphoblastoid cell line IB4, LNA-1 did not co-localize with EBNA-1, EBNA-2, EBNA-5 or EBNA-6. LNA-1 was not associated with PML containing ND10 bodies either. DNase but not RNase or detergent treatment of isolated nuclei destroys LNA-1 bodies. In advanced apoptotic cells LNA- 1 bodies remain intact but are not included in the apoptotic bodies themselves.
Biuppslag (personer, institutioner, konferenser, titlar ...)
- Kiss, C (författare)
- Mattsson, K (författare)
- Kashuba, EKarolinska Institutet (författare)
- Pokrovskaja, KKarolinska Institutet (författare)
- Juhasz, A (författare)
- Holmvall, P (författare)
- Klein, GKarolinska Institutet (författare)
- Karolinska Institutet (creator_code:org_t)
Sammanhörande titlar
- Ingår i:The Journal of general virology: Microbiology Society8080 ( Pt 11), s. 2889-29000022-13171465-2099
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- Szekely, L
- Kiss, C
- Mattsson, K
- Kashuba, E
- Pokrovskaja, K
- Juhasz, A
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- Holmvall, P
- Klein, G
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