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DASH-2: flexible, l...
DASH-2: flexible, low-cost, and high-throughput SNP genotyping by dynamic allele-specific hybridization on membrane arrays
- Article/chapterEnglish2003
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2003-05-01
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Cold Spring Harbor Laboratory,2003
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LIBRIS-ID:oai:prod.swepub.kib.ki.se:1942357
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http://kipublications.ki.se/Default.aspx?queryparsed=id:1942357URI
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https://doi.org/10.1101/gr.801103DOI
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Language:English
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Summary in:English
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Subject category:ref swepub-contenttype
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Subject category:art swepub-publicationtype
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Genotyping technologies need to be continually improved in terms of their flexibility, cost-efficiency, and throughput, to push forward genome variation analysis. To this end, we have leveraged the inherent simplicity of dynamic allele-specific hybridization (DASH) and coupled it to recent innovations of centrifugal arrays and iFRET. We have thereby created a new genotyping platform we term DASH-2, which we demonstrate and evaluate in this report. The system is highly flexible in many ways (any plate format, PCR multiplexing, serial and parallel array processing, spectral-multiplexing of hybridization probes), thus supporting a wide range of application scales and objectives. Precision is demonstrated to be in the range 99.8–100%, and assay costs are 0.05 USD or less per genotype assignment. DASH-2 thus provides a powerful new alternative for genotyping practice, which can be used without the need for expensive robotics support.
Added entries (persons, corporate bodies, meetings, titles ...)
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Howell, WM
(author)
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Stromqvist, L
(author)
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Mayr, T
(author)
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Brookes, AJ
(author)
Related titles
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In:Genome research: Cold Spring Harbor Laboratory13:5, s. 916-9241088-90511549-5469
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