WFRF:(Louhio H)
Search: WFRF:(Louhio H) > (2006) > Kit ligand and c-Ki...
- 1 of 1
- Previous record
- Next record
- To hitlist
- Carlsson, IB (author)
Kit ligand and c-Kit are expressed during early human ovarian follicular development and their interaction is required for the survival of follicles in long-term culture
- Article/chapterEnglish2006
Publisher, publication year, extent ...
- Bioscientifica,2006
Numbers
- LIBRIS-ID:oai:prod.swepub.kib.ki.se:1946186
- http://kipublications.ki.se/Default.aspx?queryparsed=id:1946186URI
- https://doi.org/10.1530/rep.1.00868DOI
Supplementary language notes
- Language:English
- Summary in:English
Part of subdatabase
- SwePubSwePub
Classification
Notes
- The receptor tyrosine c-Kit and its cognate ligand, c-Kit ligand (KL, stem cell factor, SCF), are involved in ovarian follicular development in several animal species. We studied the expression of KL and c-Kit usingin situhybridization and immunohistochemistry in donated human ovarian cortical tissue. The KL transcripts were expressed in granulosa cells of primary follicles, whereas the expression of c-Kit was confined to the oocyte and granulosa cells in primary and secondary follicles. We employed an ovarian organ culture using firstly serum-containing and then serum-free medium to study the effects of KL and an anti-c-Kit antibody, ACK2, on the development and survival of ovarian folliclesin vitro. Culture of ovarian cortical slices for 7 days resulted in a 37% increase in the number of primary follicles and a 6% increase in secondary follicles. The proportion of viable follicles decreased in all cultures. The addition of KL (1, 10 and 100 ng/ml) into the culture media did not affect the developmental stages of the follicles or the proportion of atretic follicles. Inclusion of ACK2 (800 ng/ml) in the culture medium significantly increased the proportion of atretic follicles on days 7 (49 vs 28% in control cultures) and 14 (62 vs 38%) of culture. In conclusion, c-Kit and KL are expressed in human ovaries during follicular development. Blocking the c-Kit receptor induces follicular atresia. The KL/c-Kit signaling system is likely to control the survival of human ovarian follicles during early follicular development.
Added entries (persons, corporate bodies, meetings, titles ...)
- Laitinen, MPE (author)
- Scott, JE (author)
- Louhio, H (author)
- Velentzis, L (author)
- Tuuri, T (author)
- Aaltonen, J (author)
- Ritvos, O (author)
- Winston, RML (author)
- Hovatta, OKarolinska Institutet (author)
- Karolinska Institutet (creator_code:org_t)
Related titles
- In:Reproduction (Cambridge, England): Bioscientifica131:4, s. 641-6491470-16261741-7899
Internet link
Find in a library
- Reproduction (Cambridge, England) (Search for host publication in LIBRIS)
To the university's database
- 1 of 1
- Previous record
- Next record
- To hitlist
Find more in SwePub
- By the author/editor
- Carlsson, IB
- Laitinen, MPE
- Scott, JE
- Louhio, H
- Velentzis, L
- Tuuri, T
- show more...
- Aaltonen, J
- Ritvos, O
- Winston, RML
- Hovatta, O
- show less...
- Articles in the publication
- Reproduction (Ca ...
- By the university
- Karolinska Institutet
Search outside SwePub
- Extend your search to:
- Google Book Search
- Google Scholar
Kungliga biblioteket hanterar dina personuppgifter i enlighet med EU:s dataskyddsförordning (2018), GDPR. Läs mer om hur det funkar här.
Så här hanterar KB dina uppgifter vid användning av denna tjänst.
- Copyright © LIBRIS - National Library Systems
- LIBRIS.kb.se
