WFRF:(Ameln H)
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- Gustafsson, TKarolinska Institutet (author)
VEGF-A splice variants and related receptor expression in human skeletal muscle following submaximal exercise
- Article/chapterEnglish2005
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- American Physiological Society,2005
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- LIBRIS-ID:oai:prod.swepub.kib.ki.se:1950802
- http://kipublications.ki.se/Default.aspx?queryparsed=id:1950802URI
- https://doi.org/10.1152/japplphysiol.01402.2004DOI
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- Language:English
- Summary in:English
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- VEGF-A contributes to muscle tissue angiogenesis following aerobic exercise training. The temporal response of the VEGF-A isoforms and their target receptors has not been comprehensively profiled in human skeletal muscle. We combined submaximal exercise with and without reduced leg blood flow to establish whether ischemia-induced metabolic stress was an important physiological stimuli responsible for regulating the VEGF-A system in humans. Nine healthy men performed two 45-min bouts of one-leg knee-extension exercise, with and without blood flow restriction. Muscle biopsies were obtained at rest and 2 and 6 h after exercise. Expression (mRNA) of the VEGF-A splice variants and related receptors [VEGF receptor (VEGFR)-1, VEGFR-2, and neuropilin-1] was determined by using qPCR. VEGF-Atotal expression increased more robustly after exercise with reduced blood flow, and initially this principally reflected an increase in VEGF-A165. Six hours after exercise, there was a relatively greater increase in VEGF-A189, and this response was not influenced by blood flow conditions. VEGFR-1 mRNA expression increased 2 h after exercise, and neuropilin-1 expression was transiently reduced, while all three receptors increased by 6 h. There was no evidence for the expression of the inhibitory VEGF-A165B variant in human skeletal muscle. Our study, reflecting both VEGF-A ligand and receptors, implicates metabolic perturbation as a regulator of human muscle angiogenesis and demonstrates that VEGF-A splice variants are distinctly regulated. Our findings also indicate that all three receptor genes exhibit different pretranslational regulation, in response to exercise in humans.
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- Ameln, HKarolinska Institutet (author)
- Fischer, HKarolinska Institutet (author)
- Sundberg, CJKarolinska Institutet (author)
- Timmons, JA (author)
- Jansson, EKarolinska Institutet (author)
- Karolinska Institutet (creator_code:org_t)
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- In:Journal of applied physiology (Bethesda, Md. : 1985): American Physiological Society98:6, s. 2137-21468750-75871522-1601
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