Sökning: onr:"swepub:oai:prod.swepub.kib.ki.se:1957346" >
Evaluation of serol...
Evaluation of serological methods for diagnosis of Puumala hantavirus infection (nephropathia epidemica)
-
Sjolander, KB (författare)
-
- Elgh, Fredrik, 1957- (författare)
- Umeå universitet,Virologi,Fredrik Elgh
-
KallioKokko, H (författare)
-
visa fler...
-
Vapalahti, O (författare)
-
Hagglund, M (författare)
-
Palmcrantz, V (författare)
-
Juto, P (författare)
-
Vaheri, A (författare)
-
Niklasson, B (författare)
-
- Lundkvist, A (författare)
- Karolinska Institutet
-
Kallio-Kokko, H (författare)
-
visa färre...
-
(creator_code:org_t)
- American Society for Microbiology, 1997
- 1997
- Engelska.
-
Ingår i: Journal of clinical microbiology. - : American Society for Microbiology. - 0095-1137 .- 1098-660X. ; 35:12, s. 3264-3268
- Relaterad länk:
-
https://jcm.asm.org/...
-
visa fler...
-
http://kipublication...
-
https://doi.org/10.1...
-
https://urn.kb.se/re...
-
visa färre...
Abstract
Ämnesord
Stäng
- Nephropathia epidemica (NE), Puumala (PUU) virus infection, is a febrile disease which is commonly associated with acute renal impairment. To differentiate NE from other acute febrile illnesses, a rapid and reliable serological diagnosis is important, and a number of different protocols have recently been introduced. In the present report we describe a comparative evaluation of six PUU virus immunoglobulin M (IgM) and seven IgG enzyme-linked immunosorbent assay (ELISA) protocols based on native, Escherichia coli-expressed, or baculovirus-expressed nucleocapsid protein (N). Neutralization and immunofluorescence assays were included for comparison. Equally high sensitivities and specificities were obtained with three mu-capture-based IgM ELISAs using native, baculovirus-expressed, and E. coli-expressed N antigens, respectively, and by an ELISA based on purified E. coli-expressed full-length N adsorbed to solid phase. The assays based on truncated amino-terminal N proteins, including a commercially available PUU virus IgM ELISA, all showed lower sensitivities. For detection of PUU virus-specific IgG, ELISAs based on monoclonal antibody-captured native or baculovirus-expressed N antigens showed optimal sensitivities and specificities, while the assays based on E. coli-expressed N did not detect all PUU virus IgG-positive serum samples. A commercially available PUU virus IgG ELISA based on E. coli-expressed amino-terminal N showed a significantly lower sensitivity than those of all other IgG assays.
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
Hitta via bibliotek
Till lärosätets databas
- Av författaren/redakt...
-
Sjolander, KB
-
Elgh, Fredrik, 1 ...
-
KallioKokko, H
-
Vapalahti, O
-
Hagglund, M
-
Palmcrantz, V
-
visa fler...
-
Juto, P
-
Vaheri, A
-
Niklasson, B
-
Lundkvist, A
-
Kallio-Kokko, H
-
visa färre...
- Artiklar i publikationen
-
Journal of clini ...
- Av lärosätet
-
Karolinska Institutet
-
Umeå universitet