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  • Minderjahn, J (author)

Postmitotic differentiation of human monocytes requires cohesin-structured chromatin

  • Article/chapterEnglish2022

Publisher, publication year, extent ...

  • 2022-07-25
  • Springer Science and Business Media LLC,2022

Numbers

  • LIBRIS-ID:oai:prod.swepub.kib.ki.se:235879286
  • http://kipublications.ki.se/Default.aspx?queryparsed=id:235879286URI
  • https://doi.org/10.1038/s41467-022-31892-2DOI
  • http://kipublications.ki.se/Default.aspx?queryparsed=id:153270868URI

Supplementary language notes

  • Language:English
  • Summary in:English

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Classification

  • Subject category:ref swepub-contenttype
  • Subject category:art swepub-publicationtype

Notes

  • gratis
  • Cohesin is a major structural component of mammalian genomes and is required to maintain loop structures. While acute depletion in short-term culture models suggests a limited importance of cohesin for steady-state transcriptional circuits, long-term studies are hampered by essential functions of cohesin during replication. Here, we study genome architecture in a postmitotic differentiation setting, the differentiation of human blood monocytes (MO). We profile and compare epigenetic, transcriptome and 3D conformation landscapes during MO differentiation (either into dendritic cells or macrophages) across the genome and detect numerous architectural changes, ranging from higher level compartments down to chromatin loops. Changes in loop structures correlate with cohesin-binding, as well as epigenetic and transcriptional changes during differentiation. Functional studies show that the siRNA-mediated depletion of cohesin (and to a lesser extent also CTCF) markedly disturbs loop structures and dysregulates genes and enhancers that are primarily regulated during normal MO differentiation. In addition, gene activation programs in cohesin-depleted MO-derived macrophages are disturbed. Our findings implicate an essential function of cohesin in controlling long-term, differentiation- and activation-associated gene expression programs.

Added entries (persons, corporate bodies, meetings, titles ...)

  • Fischer, A (author)
  • Maier, K (author)
  • Mendes, K (author)
  • Nuetzel, M (author)
  • Raithel, J (author)
  • Stanewsky, H (author)
  • Ackermann, U (author)
  • Månsson, RKarolinska Institutet (author)
  • Gebhard, C (author)
  • Rehli, M (author)
  • Karolinska Institutet (creator_code:org_t)

Related titles

  • In:Nature communications: Springer Science and Business Media LLC13:1, s. 4301-2041-1723

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