Differential expression patterns in chemosensory and non-chemosensory tissues of putative chemosensory genes identified by transcriptome analysis of insect pest the purple stem borer Sesamia inferens (Walker)

↓ Direkt till sidans innehåll
↓ Direkt till sidans sekundära innehåll (sidomenyn)

Search: WFRF:(Junyan Jin) > Differential expres...

2 of 2
Previous record
Next record
To hitlist

Details
MARC

Zhang, YANANNanjing Agricultural University (author)

Differential expression patterns in chemosensory and non-chemosensory tissues of putative chemosensory genes identified by transcriptome analysis of insect pest the purple stem borer Sesamia inferens (Walker)

Article/chapterEnglish2013

Publisher, publication year, extent ...

2013-07-24
Public Library of Science (PLoS),2013

Numbers

LIBRIS-ID:oai:research.chalmers.se:03d7f506-7867-473a-a2a4-1de4cbbccca8
https://doi.org/10.1371/journal.pone.0069715DOI
https://research.chalmers.se/publication/523898URI

Supplementary language notes

Language:English
Summary in:English

Part of subdatabase

SwePubSwePub

Classification

Subject category:art swepub-publicationtype
Subject category:ref swepub-contenttype

Notes

Background:A large number of insect chemosensory genes from different gene subfamilies have been identified and annotated, but their functional diversity and complexity are largely unknown. A systemic examination of expression patterns in chemosensory organs could provide important information.Methodology/Principal Findings:We identified 92 putative chemosensory genes by analysing the transcriptome of the antennae and female sex pheromone gland of the purple stem borer Sesamia inferens, among them 87 are novel in this species, including 24 transcripts encoding for odorant binding proteins (OBPs), 24 for chemosensory proteins (CSPs), 2 for sensory neuron membrane proteins (SNMPs), 39 for odorant receptors (ORs) and 3 for ionotropic receptors (IRs). The transcriptome analyses were validated and quantified with a detailed global expression profiling by Reverse Transcription-PCR for all 92 transcripts and by Quantitative Real Time RT-PCR for selected 16 ones. Among the chemosensory gene subfamilies, CSP transcripts are most widely and evenly expressed in different tissues and stages, OBP transcripts showed a clear antenna bias and most of OR transcripts are only detected in adult antennae. Our results also revealed that some OR transcripts, such as the transcripts of SNMP2 and 2 IRs were expressed in non-chemosensory tissues, and some CSP transcripts were antenna-biased expression. Furthermore, no chemosensory transcript is specific to female sex pheromone gland and very few are found in the heads.Conclusion:Our study revealed that there are a large number of chemosensory genes expressed in S. inferens, and some of them displayed unusual expression profile in non-chemosensory tissues. The identification of a large set of putative chemosensory genes of each subfamily from a single insect species, together with their different expression profiles provide further information in understanding the functions of these chemosensory genes in S. inferens as well as other insects.

Subject headings and genre

Added entries (persons, corporate bodies, meetings, titles ...)

Junyan, JinNanjing Agricultural University (author)
Rong, JinNanjing Agricultural University (author)
Xia, Yihan,1990Nanjing Agricultural University(Swepub:cth)yihanx (author)
Zhou, JingjiangRothamsted Research (author)
Deng, JianyuZhejiang Agriculture and Forestry University (author)
Dong, ShuanglinNanjing Agricultural University (author)
Nanjing Agricultural UniversityRothamsted Research (creator_code:org_t)

Related titles

In:PLoS ONE: Public Library of Science (PLoS)8:71932-62031932-6203

Internet link

Find in a library

PLoS ONE (Search for host publication in LIBRIS)

To the university's database

2 of 2
Previous record
Next record
To hitlist

Find more in SwePub

By the author/editor: Zhang, YANAN; Junyan, Jin; Rong, Jin; Xia, Yihan, 1990; Zhou, Jingjiang; Deng, Jianyu; show more...; Dong, Shuanglin; show less...

About the subject

MEDICAL AND HEALTH SCIENCES: MEDICAL AND HEAL ...; and Other Medical an ...; and Other Medical an ...

MEDICAL AND HEALTH SCIENCES: MEDICAL AND HEAL ...; and Basic Medicine; and Cell and Molecul ...

NATURAL SCIENCES: NATURAL SCIENCES; and Biological Scien ...; and Other Biological ...

Articles in the publication: PLoS ONE

By the university: Chalmers University of Technology

Search outside SwePub

Extend your search to:: Google; Google Book Search; Google Scholar

Kungliga biblioteket hanterar dina personuppgifter i enlighet med EU:s dataskyddsförordning (2018), GDPR. Läs mer om hur det funkar här.
Så här hanterar KB dina uppgifter vid användning av denna tjänst.

LIBRIS.kb.se