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The human liver-spe...
The human liver-specific proteome defined by transcriptomics and antibody-based profiling
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- Kampf, Caroline (author)
- Uppsala universitet,Molekylär och morfologisk patologi
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- Mardinoglu, Adil, 1982 (author)
- Chalmers tekniska högskola,Chalmers University of Technology
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- Fagerberg, Linn (author)
- KTH,Proteomik och nanobioteknologi,Science for Life Laboratory, SciLifeLab
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- Hallström, Björn M. (author)
- KTH,Proteomik och nanobioteknologi,Science for Life Laboratory, SciLifeLab
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- Edlund, Karolina (author)
- Uppsala universitet,Molekylär och morfologisk patologi
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- Lundberg, Emma (author)
- KTH,Proteomik och nanobioteknologi,Science for Life Laboratory, SciLifeLab
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- Pontén, Fredrik (author)
- Uppsala universitet,Molekylär och morfologisk patologi
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- Nielsen, Jens B, 1962 (author)
- KTH,Genteknologi,Science for Life Laboratory, SciLifeLab
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- Uhlén, Mathias (author)
- KTH,Proteomik och nanobioteknologi,Science for Life Laboratory, SciLifeLab
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(creator_code:org_t)
- 2014-03-19
- 2014
- English.
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In: FASEB Journal. - : Wiley. - 1530-6860 .- 0892-6638. ; 28:7, s. 2901-2914
- Related links:
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http://dx.doi.org/10...
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https://doi.org/10.1...
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Abstract
Subject headings
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- Human liver physiology and the genetic etiology of the liver diseases can potentially be elucidated through the identification of proteins with enriched expression in the liver. Here, we combined data from RNA sequencing (RNA-Seq) and antibody-based immunohistochemistry across all major human tissues to explore the human liver proteome with enriched expression, as well as the cell type-enriched expression in hepatocyte and bile duct cells. We identified in total 477 protein-coding genes with elevated expression in the liver: 179 genes have higher expression as compared to all the other analyzed tissues; 164 genes have elevated transcript levels in the liver shared with at least one other tissue type; and an additional 134 genes have a mild level of increased expression in the liver. We identified the precise localization of these proteins through antibody-based protein profiling and the subcellular localization of these proteins through immunofluorescent-based profiling. We also identified the biological processes and metabolic functions associated with these proteins, investigated their contribution in the occurrence of liver diseases, and identified potential targets for their treatment. Our study demonstrates the use of RNA-Seq and antibody-based immunohistochemistry for characterizing the human liver proteome, as well as the use of tissue-specific proteins in identification of novel drug targets and discovery of biomarkers.
Subject headings
- NATURVETENSKAP -- Biologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences (hsv//eng)
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Cell- och molekylärbiologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Cell and Molecular Biology (hsv//eng)
- NATURVETENSKAP -- Biologi -- Biokemi och molekylärbiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Biochemistry and Molecular Biology (hsv//eng)
- NATURVETENSKAP -- Biologi -- Cellbiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Cell Biology (hsv//eng)
Keyword
- HEPATOCELLULAR-CARCINOMA
- DISEASES
- HEPATITIS-C
- Cell Biology
- ATLAS
- Biology
- METABOLISM
- RNA-SEQ
- metabolism
- SERUM
- TISSUE-SPECIFIC GENES
- EXPRESSION
- RNA sequencing
- DRUGS
- Biochemistry & Molecular Biology
- immunohistochemistry
Publication and Content Type
- art (subject category)
- ref (subject category)
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To the university's database
- By the author/editor
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Kampf, Caroline
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Mardinoglu, Adil ...
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Fagerberg, Linn
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Hallström, Björn ...
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Edlund, Karolina
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Lundberg, Emma
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show more...
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Pontén, Fredrik
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Nielsen, Jens B, ...
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Uhlén, Mathias
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show less...
- About the subject
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- NATURAL SCIENCES
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NATURAL SCIENCES
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and Biological Scien ...
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- MEDICAL AND HEALTH SCIENCES
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MEDICAL AND HEAL ...
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and Basic Medicine
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and Cell and Molecul ...
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- NATURAL SCIENCES
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NATURAL SCIENCES
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and Biological Scien ...
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and Biochemistry and ...
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- NATURAL SCIENCES
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NATURAL SCIENCES
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and Biological Scien ...
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and Cell Biology
- Articles in the publication
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FASEB Journal
- By the university
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Chalmers University of Technology
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Uppsala University
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Royal Institute of Technology