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Ultra-High-Throughp...
Ultra-High-Throughput Clinical Proteomics Reveals Classifiers of COVID-19 Infection
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- Messner, Christoph B. (author)
- The Francis Crick Institute
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- Demichev, Vadim (author)
- The Francis Crick Institute,University Of Cambridge
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- Wendisch, Daniel (author)
- Charité Universitätsmedizin Berlin,Charité University Medicine Berlin
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- Michalick, Laura (author)
- Charité Universitätsmedizin Berlin,Charité University Medicine Berlin
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- White, Matthew (author)
- The Francis Crick Institute
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- Freiwald, Anja (author)
- Charité Universitätsmedizin Berlin,Charité University Medicine Berlin
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- Textoris-Taube, Kathrin (author)
- Charité Universitätsmedizin Berlin,Charité University Medicine Berlin
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- Vernardis, Spyros I. (author)
- The Francis Crick Institute
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- Egger, Anna Sophia (author)
- The Francis Crick Institute
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- Kreidl, Marco (author)
- The Francis Crick Institute
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- Ludwig, Daniela (author)
- Charité Universitätsmedizin Berlin,Charité University Medicine Berlin
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- Kilian, Christiane (author)
- Charité Universitätsmedizin Berlin,Charité University Medicine Berlin
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- Agostini, Federica (author)
- Charité Universitätsmedizin Berlin,Charité University Medicine Berlin
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- Zelezniak, Aleksej, 1984 (author)
- The Francis Crick Institute,Chalmers tekniska högskola,Chalmers University of Technology
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- Thibeault, Charlotte (author)
- Charité Universitätsmedizin Berlin,Charité University Medicine Berlin
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- Pfeiffer, Moritz (author)
- Charité Universitätsmedizin Berlin,Charité University Medicine Berlin
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- Hippenstiel, Stefan (author)
- Charité Universitätsmedizin Berlin,Charité University Medicine Berlin
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- Hocke, Andreas (author)
- Charité Universitätsmedizin Berlin,Charité University Medicine Berlin
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- von Kalle, Christof (author)
- Charité Universitätsmedizin Berlin,Charité University Medicine Berlin
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- Campbell, Archie (author)
- University of Edinburgh
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- Hayward, Caroline (author)
- University of Edinburgh
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- Porteous, David J. (author)
- University of Edinburgh
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- Marioni, Riccardo E. (author)
- University of Edinburgh
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- Langenberg, C. (author)
- University Of Cambridge,The Francis Crick Institute
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- Lilley, Kathryn S. (author)
- University Of Cambridge
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- Kuebler, Wolfgang M. (author)
- Charité Universitätsmedizin Berlin,Charité University Medicine Berlin
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- Mülleder, Michael (author)
- Charité Universitätsmedizin Berlin,Charité University Medicine Berlin
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- Drosten, C. (author)
- Charité Universitätsmedizin Berlin,Charité University Medicine Berlin
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- Suttorp, Norbert (author)
- Charité Universitätsmedizin Berlin,Charité University Medicine Berlin
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- Witzenrath, Martin (author)
- Charité Universitätsmedizin Berlin,Charité University Medicine Berlin
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- Kurth, Florian (author)
- Charité Universitätsmedizin Berlin,Charité University Medicine Berlin
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- Sander, Leif Erik (author)
- Charité Universitätsmedizin Berlin,Charité University Medicine Berlin
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- Ralser, M. (author)
- The Francis Crick Institute,Charité Universitätsmedizin Berlin,Charité University Medicine Berlin
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(creator_code:org_t)
- Elsevier BV, 2020
- 2020
- English.
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In: Cell Systems. - : Elsevier BV. - 2405-4712 .- 2405-4720. ; 11:1, s. 11-24.E4
- Related links:
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https://research.cha... (primary) (free)
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http://www.cell.com/...
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https://research.cha...
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https://doi.org/10.1...
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Abstract
Subject headings
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- The COVID-19 pandemic is an unprecedented global challenge, and point-of-care diagnostic classifiers are urgently required. Here, we present a platform for ultra-high-throughput serum and plasma proteomics that builds on ISO13485 standardization to facilitate simple implementation in regulated clinical laboratories. Our low-cost workflow handles up to 180 samples per day, enables high precision quantification, and reduces batch effects for large-scale and longitudinal studies. We use our platform on samples collected from a cohort of early hospitalized cases of the SARS-CoV-2 pandemic and identify 27 potential biomarkers that are differentially expressed depending on the WHO severity grade of COVID-19. They include complement factors, the coagulation system, inflammation modulators, and pro-inflammatory factors upstream and downstream of interleukin 6. All protocols and software for implementing our approach are freely available. In total, this work supports the development of routine proteomic assays to aid clinical decision making and generate hypotheses about potential COVID-19 therapeutic targets.
Subject headings
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinsk bioteknologi -- Biomedicinsk laboratorievetenskap/teknologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Medical Biotechnology -- Biomedical Laboratory Science/Technology (hsv//eng)
- NATURVETENSKAP -- Biologi -- Mikrobiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Microbiology (hsv//eng)
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinsk bioteknologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Medical Biotechnology (hsv//eng)
- NATURVETENSKAP -- Data- och informationsvetenskap -- Programvaruteknik (hsv//swe)
- NATURAL SCIENCES -- Computer and Information Sciences -- Software Engineering (hsv//eng)
- TEKNIK OCH TEKNOLOGIER -- Elektroteknik och elektronik -- Datorsystem (hsv//swe)
- ENGINEERING AND TECHNOLOGY -- Electrical Engineering, Electronic Engineering, Information Engineering -- Computer Systems (hsv//eng)
Keyword
- antiviral immune response
- SWATH-MS
- clinical classifiers
- high-throughput proteomics
- COVID-19 infection
- mass spectrometry
Publication and Content Type
- art (subject category)
- ref (subject category)
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- By the author/editor
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Messner, Christo ...
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Demichev, Vadim
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Wendisch, Daniel
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Michalick, Laura
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White, Matthew
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Freiwald, Anja
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Textoris-Taube, ...
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Vernardis, Spyro ...
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Egger, Anna Soph ...
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Kreidl, Marco
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Ludwig, Daniela
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Kilian, Christia ...
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Agostini, Federi ...
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Zelezniak, Aleks ...
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Thibeault, Charl ...
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Pfeiffer, Moritz
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Hippenstiel, Ste ...
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Hocke, Andreas
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von Kalle, Chris ...
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Campbell, Archie
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Hayward, Carolin ...
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Porteous, David ...
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Marioni, Riccard ...
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Langenberg, C.
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Lilley, Kathryn ...
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Kuebler, Wolfgan ...
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Mülleder, Michae ...
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Drosten, C.
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Suttorp, Norbert
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Witzenrath, Mart ...
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Kurth, Florian
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Sander, Leif Eri ...
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Ralser, M.
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- About the subject
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- MEDICAL AND HEALTH SCIENCES
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MEDICAL AND HEAL ...
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and Medical Biotechn ...
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and Biomedical Labor ...
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- NATURAL SCIENCES
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NATURAL SCIENCES
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and Biological Scien ...
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and Microbiology
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- MEDICAL AND HEALTH SCIENCES
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MEDICAL AND HEAL ...
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and Medical Biotechn ...
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- NATURAL SCIENCES
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NATURAL SCIENCES
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and Computer and Inf ...
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and Software Enginee ...
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- ENGINEERING AND TECHNOLOGY
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ENGINEERING AND ...
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and Electrical Engin ...
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and Computer Systems
- Articles in the publication
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Cell Systems
- By the university
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Chalmers University of Technology